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39450-01-6 - Proteinase K, Tritirachium album limber - J62051 - Alfa Aesar

J62051 Proteinase K, Tritirachium album limber

CAS Number

Size Price ($) Quantity Availability
25mg 49.75
100mg 106.09
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Proteinase K, Tritirachium album limber


Chemical Properties

Lyophilized powder, 28.9 kDa
It is fully miscible with water.


Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of Proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification. Proteinase K is specifically formulated for molecular cloning, nucleic acid research and protein/peptide structural analysis, inactivation of Rnase and Dnases, isolation of high molecular weight DNA, isolation of RNA or the isolation of plasmid and genomic DNA. Proteinase K is used for the isolation of native high molecular genomic nucleic acids.


Minimum 20 units per mg dry weight. One unit releases one micromole of Folin positive amino acids, measured as tyrosine, at 37 C and pH 7.5, using urea denatured hemoglobin as the substrate

Literature References

Betzel C.; Singh TP.; Visanji M.; Peters K.; Fittkau S.; Saenger W.; Wilson KS. Structure of the complex of proteinase K with a substrate analogue hexapeptide inhibitor at 2.2-A resolution.   J. Biol. Chem. 1993, 268, (21), 15854-8.

Müller A.; Hinrichs W.; Wolf WM.; Saenger W. Crystal structure of calcium-free proteinase K at 1.5-A resolution. J. Biol. Chem. 1994, 269, (37), 23108-11.

GHS Hazard and Precautionary Statements

Hazard Statements: H334-H315-H319-H335

May cause allergy or asthma symptoms or breathing difficulties if inhaled. Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.

Precautionary Statements: P285-P261-P280-P305+P351+P338-P304+P340-P342+P311-P362-P312-P405-P501a

In case of inadequate ventilation wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician. Take off contaminated clothing and wash before reuse. Call a POISON CENTER or doctor/physician if you feel unwell. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Other References

Harmonized Tariff Code


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