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5625-37-6 - PIPES, 1.0M buffer soln., pH 7.0 - J62195 - Alfa Aesar

J62195 PIPES, 1.0M buffer soln., pH 7.0

CAS Number

Size Price ($) Quantity Availability
250ml 96.41
500ml 160.68
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PIPES, 1.0M buffer soln., pH 7.0


Chemical Properties

Soluble in 1M NaOH (0.5 M at 20°C), water (~1 g/L at 100°C), and 0.5 N NaOH (0.1 M).


PIPES (Piperazine-N,N?-bis(2-ethanesulfonic acid), is frequently used as a buffering agent in biochemistry. PIPES has pKa near the physiological pH which makes it useful in cell culture work. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues and has a negligible capacity to bind divalent ions. Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer is done.


Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Keep away from strong oxidizing agents.

Literature References

MJ Alowitz.; MM Scherer. Kinetics of nitrate, nitrite, and Cr (VI) reduction by iron metal. Environmental Science & Technology.2002, 36, (3), 299-306.

JB Olmsted, GG Borisy. Ionic and nucleotide requirements for microtubule polymerization in vitro. Biochemistry. 1975, 14, (13), 2996-3005.

Other References

Harmonized Tariff Code


  • A16086

    Ethylene glycol-O,O'-bis(2-aminoethyl)-N,N,N',N'-tetraacetic acid, 97%
  • B21835

    PIPES monosodium salt hydrate, 99%
  • J60618

    PIPES, 1.0M buffer soln., pH 8.0
  • J60659

    PIPES, 1.0M buffer soln., pH 6.5
  • J61556

    MES, 1.0M buffer soln., pH 8.0

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