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3,3?-diaminobenzidine-tetrahydrochloride-dihydrate (DAB) was used as a chromogen, for detecting the antigen-antibody complex. It is one of the most potent marker being used in immunochemistry, mostly at the electron and light microscope levels. It is used as a histological stain for peroxidase activity. Reaction between 3,3'-Diaminobenzidine and peroxidase forms a visible insoluble product. This approach has been used to assay activity of glutamate decarboxylase in rat cerebellar sections and to detect horseradish peroxidase presence on cultured cell surfaces.
Roschzttardtz, H.; Grillet, L.; Isaure, M.-P.; Conejero, G.; Ortega, R.; Curie, C.; Mari, S. The Plant Cell Nucleolus as a Hot Spot for Iron. The Journal of Biological Chemistry . 2011, 286, (32), 27863-27866.
JK Stille.; JR Williamson. Polyquinoxalines. Journal of Polymer Science Part A 1964, 2, (9), 3867-3875.
Hazard Statements: H315-H319-H351-H335
Causes skin irritation. Causes serious eye irritation. Suspected of causing cancer. May cause respiratory irritation.
Precautionary Statements: P201-P261-P280-P305+P351+P338-P405-P501a
Obtain special instructions before use. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.