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3,3?-diaminobenzidine-tetrahydrochloride-dihydrate (DAB) was used as a chromogen, for detecting the antigen-antibody complex. It is one of the most potent marker being used in immunochemistry, mostly at the electron and light microscope levels. It is used as a histological stain for peroxidase activity. Reaction between 3,3'-Diaminobenzidine and peroxidase forms a visible insoluble product. This approach has been used to assay activity of glutamate decarboxylase in rat cerebellar sections and to detect horseradish peroxidase presence on cultured cell surfaces.
Roschzttardtz, H.; Grillet, L.; Isaure, M.-P.; Conejero, G.; Ortega, R.; Curie, C.; Mari, S. The Plant Cell Nucleolus as a Hot Spot for Iron. The Journal of Biological Chemistry . 2011, 286, (32), 27863-27866.
JK Stille.; JR Williamson. Polyquinoxalines. Journal of Polymer Science Part A 1964, 2, (9), 3867-3875.
Hazard Statements: H315-H320-H335-H341-H350
Causes skin irritation. Causes eye irritation. May cause respiratory irritation. Suspected of causing genetic defects. May cause cancer.
Precautionary Statements: P201-P202-P264b-P271-P280-P302+P352-P304+P340-P305+P351+P338-P308+P313-P332+P313-P362-P501c
Obtain special instructions before use. Do not handle until all safety precautions have been read and understood. Wash face, hands and any exposed skin thoroughly after handling Use only outdoors or in a well-ventilated area. Wear protective gloves/protective clothing/eye protection/face protection. IF ON SKIN: Wash with plenty of soap and water. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF exposed or concerned: Get medical advice/attention. If skin irritation occurs: Take off contaminated clothing and wash before reuse. Dispose of contents/ container to an approved waste disposal plant