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868272-85-9 - 3,3'-Diaminobenzidine tetrahydrochloride hydrate - DAB tetrahydrochloride hydrate - J62216 - Alfa Aesar

J62216 3,3'-Diaminobenzidine tetrahydrochloride hydrate

CAS Number
DAB tetrahydrochloride hydrate

Size Price ($) Quantity Availability
5g 77.35
10g 130.81
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3,3'-Diaminobenzidine tetrahydrochloride hydrate


Chemical Properties

Formula Weight
Soluble at 5%(w/v) in water


3,3?-diaminobenzidine-tetrahydrochloride-dihydrate (DAB) was used as a chromogen, for detecting the antigen-antibody complex. It is one of the most potent marker being used in immunochemistry, mostly at the electron and light microscope levels. It is used as a histological stain for peroxidase activity. Reaction between 3,3'-Diaminobenzidine and peroxidase forms a visible insoluble product. This approach has been used to assay activity of glutamate decarboxylase in rat cerebellar sections and to detect horseradish peroxidase presence on cultured cell surfaces.


Store at room temperature. The powder has a shelf-life of 3 years. Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Light sensitive. hygroscopic Store with desiccant. Handle under nitrogen, protect from moisture. Store under nitrogen

Literature References

Roschzttardtz, H.; Grillet, L.; Isaure, M.-P.; Conejero, G.; Ortega, R.; Curie, C.; Mari, S. The Plant Cell Nucleolus as a Hot Spot for Iron. The Journal of Biological Chemistry . 2011, 286, (32), 27863-27866.

JK Stille.; JR Williamson. Polyquinoxalines. Journal of Polymer Science Part A 1964, 2, (9), 3867-3875.

GHS Hazard and Precautionary Statements

Hazard Statements: H351-H315-H319-H335

Suspected of causing cancer. Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.

Precautionary Statements: P201-P261-P280-P281-P305+P351+P338-P304+P340-P362-P312-P405-P501a

Obtain special instructions before use. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. Use personal protective equipment as required. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. Take off contaminated clothing and wash before reuse. Call a POISON CENTER or doctor/physician if you feel unwell. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Other References

Harmonized Tariff Code


  • A14331

    5,5'-Dithiobis(2-nitrobenzoic acid), 99%
  • B21932

    Crystal Violet
  • L13376

    4-Nitrophenyl-alpha-D-galactopyranoside, 98+%

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