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Deoxyribonuclease I is used for the removal of DNA from protein samples. DNAse I is used to nick DNA as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used during the isolation of plasma membrane vesicles from Neurospora crassa cell culture.1 It has also been used along with trypsin for the preparation of single cell suspension from rat testes. Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis
G A Scarborough. The neurospora plasma membrane ATPase is an electrogenic pump. PNAS. 1976, 73 (5), 1485-1488.
U Lindberg. Molecular weight and amino acid composition of deoxyribonuclease I. Biochemistry. 1967, 6 (1), 335-342.
Hazard Statements: H317-H334
May cause an allergic skin reaction. May cause allergy or asthma symptoms or breathing difficulties if inhaled.
Precautionary Statements: P272-P280g-P285-P302+P352-P304+P341-P333+P313-P342+P311-P363-P501c
Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves. In case of inadequate ventilation wear respiratory protection. IF ON SKIN: Wash with plenty of soap and water. IF INHALED: If breathing is difficult, remove to fresh air and keep at rest in a position comfortable for breathing. If skin irritation or rash occurs: Get medical advice/attention. If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician. Wash contaminated clothing before reuse. Dispose of contents/ container to an approved waste disposal plant