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9003-98-9 - Deoxyribonuclease I, bovine pancreas - EC 3.1.21.1 - DNase I - J62229 - Alfa Aesar

J62229 Deoxyribonuclease I, bovine pancreas

CAS Number
9003-98-9
Synonyms
EC 3.1.21.1
DNase I

Size Price ($) Quantity Availability
25mg 53.56
100mg 139.05
1g 938.53
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Deoxyribonuclease I, bovine pancreas

MDL
MFCD00130918
EINECS
232-667-0

Chemical Properties

Form
Lyophilized powder, 31 kDa
Solubility
It is soluble in 0.15 M NaCl.

Applications

Deoxyribonuclease I is used for the removal of DNA from protein samples. DNAse I is used to nick DNA as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used during the isolation of plasma membrane vesicles from Neurospora crassa cell culture.1 It has also been used along with trypsin for the preparation of single cell suspension from rat testes. Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis

Notes

Minimum 2,000 units per mg dry weight. One unit causes an increase in absorbance at 260nm of 0.001 per minute per ml at 25 degrees when acting upon highly polymerized DNA at pH 5.0

Literature References

G A Scarborough. The neurospora plasma membrane ATPase is an electrogenic pump. PNAS. 1976, 73 (5), 1485-1488.

U Lindberg. Molecular weight and amino acid composition of deoxyribonuclease I. Biochemistry. 1967, 6 (1), 335-342.

Other References

Merck
14,2906
Harmonized Tariff Code
3507.90
TSCA
Yes
RTECS
RF0750000

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