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9001-99-4 - Ribonuclease A, bovine pancreas, Molecular Biology Grade - EC 3.1.27.5 - Rnase A - J62232 - Alfa Aesar

J62232 Ribonuclease A, bovine pancreas, Molecular Biology Grade

CAS Number
9001-99-4
Synonyms
EC 3.1.27.5
Rnase A

Size Price ($) Quantity Availability
200mg 110.00
1g 440.00
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Ribonuclease A, bovine pancreas, Molecular Biology Grade

MDL
MFCD00132181
EINECS
232-646-6

Chemical Properties

Form
Lyophilized powder
Sensitivity
Moisture Sensitive
Solubility
Reconstitute in 10mM acetate, pH 5.0 with or without 15mM calcium chloride

Applications

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess hybridase activity of human ribonuclease-1. Ribonuclease A from bovine pancreas has also been used in a study to investigate particle-based and monolithic columns for cation exchange protein displacement chromatography.

Notes

Minimum 2500 units/mg dry wt. Chromatographically purified. Heat treat at 100°C for 15 minutes to inactivate DNAase. One unit causes an increase in absorbance of 1.0 at 260nm at 37 C and pH 5.0 when yeast ribosomal RNA is hydrolyzed to acid soluble oligonucleotides.

Literature References

Nana Asare et. al. 1-Nitropyrene (1-NP) induces apoptosis and apparently a non-apoptotic programmed cell death (paraptosis) in Hepa1c1c7 cells. Toxicology and Applied Pharmacology. 2008, 230, (2), 175-186.

W Norde.; J Lyklema. The adsorption of human plasma albumin and bovine pancreas ribonuclease at negatively charged polystyrene surfaces: I. Adsorption isotherms. Effects of charge, ionic strength, and temperature. Journal of Colloid and Interface Science. 1978, 66, (2), 257-265.

Other References

Merck
14,8202
Harmonized Tariff Code
3507.90
TSCA
Yes
RTECS
RF0760000

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