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Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids.1,2,3 It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA.4 Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.
S P Moore and B M Sutherland. A densitometric nondestructive microassay for DNA quantitation. Analytical Biochemistry. 1985, 144(1), 15-19.
A R El Hamalawi et. al. The fluorometric determination of nucleic acids in pea seeds by use of ethidium bromide complexes. Analytical Biochemistry. 1975, 67(2), 384-391.
Hazard Statements: H333-H341
May be harmful if inhaled. Suspected of causing genetic defects.
Precautionary Statements: P201-P280-P304+P312a-P308+P313-P405-P501a
Obtain special instructions before use. Wear protective gloves/protective clothing/eye protection/face protection. IF INHALED: IF exposed or concerned: Get medical advice/attention. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.