TSS buffer is used to make chemically competent cells. TSS solution is used in the one-step procedure for the preparation of competent Escherichia coli and allows transformation of cells without heat-shock. ransformation efficiencies of 106-108/µg DNA are typically obtained using TSS.
Ravi S. Kamath.; Julie Ahringer. Genome-wide RNAi screening in Caenorhabditis elegans.Methods. 2003, 30 (4),313-321 .