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J62318 TSS (Transformation & Storage Soln.), pH 6.5

CAS Number
Synonyms

Stock No. Size Price ($) Quantity Availability
J62318-=E 125ml 47.30
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TSS (Transformation & Storage Soln.), pH 6.5

Chemical Properties

Form
Liquid
Storage & Sensitivity
Ambient temperatures.
Solubility
Fully miscible in water.

Applications

TSS buffer is used to make chemically competent cells. TSS solution is used in the one-step procedure for the preparation of competent Escherichia coli and allows transformation of cells without heat-shock. ransformation efficiencies of 106-108/µg DNA are typically obtained using TSS.

Notes

Contains 85% LB medium, 10% (w/v) PEG MW 8000, 5% (v/v) DMSO, 50mM magnesium chloride , pH 6.5, sterile filtered.

Literature References

Ravi S. Kamath.; Julie Ahringer. Genome-wide RNAi screening in Caenorhabditis elegans.Methods. 2003, 30 (4),313-321 .

Other References

Harmonized Tariff Code
3822.00
TSCA
No

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