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Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gels sample well.
Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component. Glycerol acts as a cryoprotectant and prevents formation of ice crystals and prevents cell damage. Glycerol is useful in a variety of applications, glycerol is used as a solvent, plasticizer and humectant.
In polyacrylamide gel electrophoresis, glycerol is used in sample preparation and gel formation. At a concentration of 5-10%, glycerol is used to increase the density of a sample so that it will layer at the bottom of a sample well. Additional uses include as an aid in casting gradient gels, a protein stabilizer and storage buffer component.
Jenkins, B. T.; Hajra, A. K. Glycerol Kinase and Dihydroxyacetone Kinase in Rat Brain. Journal of Neurochemistry . 1976, 26(2), 377-385.
Tildon, J. T.; Stevenson Jr, J. H.; Ozand, P. T. Mitochondrial glycerol kinase activity in rat brain. The Biochemical journal . 1976, 157(2), 513-6.