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Denhardt's solution (100X) - J62416 - Alfa Aesar

J62416 Denhardt's solution (100X)

CAS Number

Size Price ($) Quantity Availability
50ml 96.41
100ml 124.80
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Denhardt's solution (100X)


Chemical Properties

Soluble in water.


Denhardt's Solution is used as a blocking reagent for northern or southern blots to prevent probe binding on open membrane space and non-target nucleic acids. Denhardt's reagent is recommended for Northern hybridization, hybridization of RNA, single copy Southern hybridization' or hybridization of DNA, immobilized on nylon membranes.


2% Ficoll (Type 400, Pharmacia), 2% polyvinylpyrrolidone and 2% bovine serum albumin

Literature References

MJ Zoller.; M Smith. Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA. span class="italictxt"> Nucleic Acids Research. 1982, 10, (20), 6487-6500.

A Kaipainen.; J Korhonen.; K Pajusola. The related FLT4, FLT1, and KDR receptor tyrosine kinases show distinct expression patterns in human fetal endothelial cells. span class="italictxt"> Nucleic Acids Research. 1993, 178, (6), 2077-2088.

Other References

Harmonized Tariff Code


  • A16046

    Triton® X-100
  • J61038

    TRIS, 1.0M buffer soln., pH 8.5, 0.2 micron filtered
  • J61429

    TRIS, 1.0M buffer soln., pH 8.0, 0.2 micron filtered
  • J62993

    TRIS, 1.0M buffer soln., pH 7.5, 0.2 micron filtered
  • J60561

    SSC (20X), RNAse free

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