Denhardt's Solution is used as a blocking reagent for northern or southern blots to prevent probe binding on open membrane space and non-target nucleic acids. Denhardt's reagent is recommended for Northern hybridization, hybridization of RNA, single copy Southern hybridization' or hybridization of DNA, immobilized on nylon membranes.
MJ Zoller.; M Smith. Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA. span class="italictxt"> Nucleic Acids Research. 1982, 10, (20), 6487-6500.
A Kaipainen.; J Korhonen.; K Pajusola. The related FLT4, FLT1, and KDR receptor tyrosine kinases show distinct expression patterns in human fetal endothelial cells. span class="italictxt"> Nucleic Acids Research. 1993, 178, (6), 2077-2088.