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1135-40-6 - CAPS, 0.5M buffer soln., pH 10.5 - J62446 - Alfa Aesar

J62446 CAPS, 0.5M buffer soln., pH 10.5

CAS Number
1135-40-6
Synonyms

Size Price ($) Quantity Availability
250ml 56.24
500ml 88.37
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CAPS, 0.5M buffer soln., pH 10.5

MDL
MFCD00003837

Chemical Properties

Form
Liquid
Solubility
Soluble in water (100 g/L) at 20°C, and methanol (1%).

Applications

CAPS (N-cyclohexyl-3-aminopropanesulfonic acid) buffer salt is used to formulate CAPS buffer, a zwitterionic buffer that is useful in the range of pH 7.9-11.1. CAPS buffer is widely used in Western and immunoblotting experiments as well as protein sequencing and identification. Used in the electrotransfer of proteins to PVDF (sc-3723) or nitrocellulose membranes (sc-3718, sc-3724). The high pH of this buffer makes it useful for the transfer of proteins with a pI > 8.5. CAPS is not one of the original Good’s buffers, although it has a similar structure to the other propanesulfonic acids and was selected as a highly water soluble buffering reagent with an optimum buffering pH of 10.4 and minimal reactivity with enzymes or proteins, minimal salt effects.

Notes

Store at room temperature. Ensure good ventilation. Incompatible with oxidizing agents.

Literature References

Marion J. Sheltawy.; M.S. Losowsky. Determination of faecal bile acids by an enzymic method. Clin. Chim. Acta. 1975, 64 (2),127-132 .

Other References

Harmonized Tariff Code
2921.30
TSCA
Yes

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