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1135-40-6 - CAPS, 0.5M buffer soln., pH 10.5 - J62446 - Alfa Aesar

J62446 CAPS, 0.5M buffer soln., pH 10.5

CAS Number

Size Price ($) Quantity Availability
250ml 55.70
500ml 87.50
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CAPS, 0.5M buffer soln., pH 10.5


Chemical Properties

Soluble in water (100 g/L) at 20°C, and methanol (1%).


CAPS (N-cyclohexyl-3-aminopropanesulfonic acid) buffer salt is used to formulate CAPS buffer, a zwitterionic buffer that is useful in the range of pH 7.9-11.1. CAPS buffer is widely used in Western and immunoblotting experiments as well as protein sequencing and identification. Used in the electrotransfer of proteins to PVDF (sc-3723) or nitrocellulose membranes (sc-3718, sc-3724). The high pH of this buffer makes it useful for the transfer of proteins with a pI > 8.5. CAPS is not one of the original Good’s buffers, although it has a similar structure to the other propanesulfonic acids and was selected as a highly water soluble buffering reagent with an optimum buffering pH of 10.4 and minimal reactivity with enzymes or proteins, minimal salt effects.


Store at room temperature. Ensure good ventilation. Incompatible with oxidizing agents.

Literature References

Marion J. Sheltawy.; M.S. Losowsky. Determination of faecal bile acids by an enzymic method. Clin. Chim. Acta. 1975, 64 (2),127-132 .

Other References

Harmonized Tariff Code


  • J60024

    Citrate, 0.5M buffer soln., pH 4.5
  • J61413

    Potassium phosphate, 0.5M buffer soln., pH 7.4
  • J62131

    TRIS, 0.5M buffer soln., pH 8.5
  • J62261

    MOPS, 1.0M buffer soln., pH 5.5
  • J62333

    CHES, 0.5M buffer soln, pH 9.5

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