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J62446 CAPS, 0.5M buffer soln., pH 10.5

CAS Number
1135-40-6
Synonyms

Stock No. Size Price ($) Quantity Availability
J62446-AK 250ml 58.00
J62446-AP 500ml 91.00
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CAPS, 0.5M buffer soln., pH 10.5

MDL
MFCD00003837

Chemical Properties

Form
Liquid
Storage & Sensitivity
Ambient temperatures.
Solubility
Soluble in water (100 g/L) at 20°C, and methanol (1%).

Applications

CAPS (N-cyclohexyl-3-aminopropanesulfonic acid) buffer salt is used to formulate CAPS buffer, a zwitterionic buffer that is useful in the range of pH 7.9-11.1. CAPS buffer is widely used in Western and immunoblotting experiments as well as protein sequencing and identification. Used in the electrotransfer of proteins to PVDF (sc-3723) or nitrocellulose membranes (sc-3718, sc-3724). The high pH of this buffer makes it useful for the transfer of proteins with a pI > 8.5. CAPS is not one of the original Good’s buffers, although it has a similar structure to the other propanesulfonic acids and was selected as a highly water soluble buffering reagent with an optimum buffering pH of 10.4 and minimal reactivity with enzymes or proteins, minimal salt effects.

Notes

Store at room temperature. Ensure good ventilation. Incompatible with oxidizing agents.

Literature References

Marion J. Sheltawy.; M.S. Losowsky. Determination of faecal bile acids by an enzymic method. Clin. Chim. Acta. 1975, 64 (2),127-132 .

GHS Hazard and Precautionary Statements

Hazard Statements: H315-H319

Causes skin irritation. Causes serious eye irritation.

Precautionary Statements: P264b-P280-P302+P352-P305+P351+P338-P332+P313-P337+P313-P362

Wash face, hands and any exposed skin thoroughly after handling Wear protective gloves/protective clothing/eye protection/face protection. IF ON SKIN: Wash with plenty of soap and water. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If skin irritation occurs: Get medical advice/attention. If eye irritation persists: Take off contaminated clothing and wash before reuse.

Other References

Harmonized Tariff Code
2921.30
TSCA
Yes

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