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TBE (10X), TRIS + borate + EDTA - J62449 - Alfa Aesar

J62449 TBE (10X), TRIS + borate + EDTA

CAS Number
Synonyms

Size Price ($) Quantity Availability
1L 27.30
2L 37.44
4L 88.37
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TBE (10X), TRIS + borate + EDTA

MDL
MFCD00236358

Chemical Properties

Form
Liquid
Solubility
It is soluble in water.

Applications

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. TBE Buffer is used for polyacrylamide and agarose gel electrophoresis. It is ideally suited for DNA and RNA separation on longer run, higher voltage or amperage gels. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel.5 TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Tris-Borate-EDTA buffer has been used for pulsed-field gel electrophoresis (PFGE).

Notes

0.9M Tris base, 0.9M boric acid, 20mM EDTA

Literature References

Brody, J.R.; Kern, S.E. History and principles of conductive media for standard DNA electrophoresis. Anal Biochem . 2004, 333, (1), 1-13.

L Orbán and A Chrambach. Discontinuous buffer system for polyacrylamide and agarose gel electrophoresis of DNA fragments. Electrophoresis. 1991, 12, (4), 233-240.

GHS Hazard and Precautionary Statements

Hazard Statements: H360-H315-H319

May damage fertility or the unborn child. Causes skin irritation. Causes serious eye irritation.

Precautionary Statements: P201-P280-P281-P305+P351+P338-P362-P308+P313-P321-P332+P313-P405-P501a

Obtain special instructions before use. Wear protective gloves/protective clothing/eye protection/face protection. Use personal protective equipment as required. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Take off contaminated clothing and wash before reuse. IF exposed or concerned: Get medical advice/attention. Specific treatment (see label). If skin irritation occurs: Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Other References

Harmonized Tariff Code
3822.00
TSCA
Yes

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