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5625-37-6 - PIPES, 1.0M buffer soln., pH 7.5 - J62494 - Alfa Aesar

J62494 PIPES, 1.0M buffer soln., pH 7.5

CAS Number
5625-37-6
Synonyms

Size Price ($) Quantity Availability
250ml 96.41
500ml 160.68
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PIPES, 1.0M buffer soln., pH 7.5

MDL
MFCD00006159

Chemical Properties

Form
Liquid
Solubility
Soluble in 1M NaOH (0.5 M at 20°C), water (~1 g/L at 100°C), and 0.5 N NaOH (0.1 M).

Applications

PIPES (Piperazine-N,N?-bis(2-ethanesulfonic acid), is frequently used as a buffering agent in biochemistry. PIPES has pKa near the physiological pH which makes it useful in cell culture work. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues and has a negligible capacity to bind divalent ions. Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer is done.

Notes

Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Keep away from strong oxidizing agents.

Literature References

MJ Alowitz.; MM Scherer. Kinetics of nitrate, nitrite, and Cr (VI) reduction by iron metal. Environmental Science & Technology.2002, 36, (3), 299-306.

JB Olmsted, GG Borisy. Ionic and nucleotide requirements for microtubule polymerization in vitro. Biochemistry. 1975, 14, (13), 2996-3005.

Other References

Harmonized Tariff Code
2933.59
TSCA
Yes

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