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RIPA buffer is used to lyse cells and tissues. It is used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. This buffer enables protein extraction from cytoplasmic, membrane and and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts. It is compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification. This product is ideal for general cell-tissue lysis and sub cellular organelle or molecular fractionation, including immunoprecipation.
MS Mccoy.; CI Bargmann. Human colon carcinoma Ki-ras2 oncogene and its corresponding proto-oncogene. Mol. Cell. Biol. 1984, 4, (8), 1577-1582.
T Dorfman.; A Bukovsky.; A Ohagen.; S Höglund. Functional domains of the capsid protein of human immunodeficiency virus type 1. J. Virol. 1994, 68, (12), 8180-8187.
Hazard Statements: H318-H335
Causes serious eye damage. May cause respiratory irritation.
Precautionary Statements: P280-P305+P351+P338-P310
Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician.