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J62524 RIPA buffer (5X)

CAS Number

Stock No. Size Price ($) Quantity Availability
J62524-AD 50ml 63.30
J62524-AE 100ml 109.00
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RIPA buffer (5X)


Chemical Properties

Storage & Sensitivity
Keep Cold.
It is soluble in water.


RIPA buffer is used to lyse cells and tissues. It is used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. This buffer enables protein extraction from cytoplasmic, membrane and and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts. It is compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification. This product is ideal for general cell-tissue lysis and sub cellular organelle or molecular fractionation, including immunoprecipation.


250mM Tris-HCl (pH 7.4), 750mM NaCl, 5% NP-40, 2.5% sodium deoxycholate,0.5% SDS.

Literature References

MS Mccoy.; CI Bargmann. Human colon carcinoma Ki-ras2 oncogene and its corresponding proto-oncogene. Mol. Cell. Biol. 1984, 4, (8), 1577-1582.

T Dorfman.; A Bukovsky.; A Ohagen.; S Höglund. Functional domains of the capsid protein of human immunodeficiency virus type 1. J. Virol. 1994, 68, (12), 8180-8187.

GHS Hazard and Precautionary Statements

Hazard Statements: H318-H335

Causes serious eye damage. May cause respiratory irritation.

Precautionary Statements: P280-P305+P351+P338-P310

Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician.

Other References

Harmonized Tariff Code


  • A15797

    1,4-Dithio-DL-threitol, 98%
  • B24026

    4-(Trifluoromethylthio)benzyl alcohol, 97%
  • J60143

    NP-40 lysis buffer with glycerol (2X)
  • J60568

    PIPES lysis buffer with NP-40 (2X)
  • J60015

    Laemmli SDS sample buffer, reducing (4X)

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