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Glycine is widely used as a buffer for a variety of immunological applications. It is frequently used in the preparation of TG Buffers where the buffer is used as a running and/or transfer buffer for polyacrylamide gel electrophoresis and western blotting. Glycine is also used as an eluent in column chromatography. Glycine has been reported for use in the enzymatic determination of lactate using lactic dehydrogenase.
H Towbin, et al. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proc Natl Acad Sci U S A.,1979,76(9), 4350-4354.
Laemmli UK, et al. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature.,1970,227(5259), 680-5.