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J62527 Glycine, 0.2M buffer soln., pH 3.0

CAS Number

Stock No. Size Price ($) Quantity Availability
J62527-AK 250ml 50.20
J62527-AP 500ml 75.20
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Glycine, 0.2M buffer soln., pH 3.0


Chemical Properties

Storage & Sensitivity
Ambient temperatures.
Not miscible or difficult to mix in water. Slightly soluble in acetone. Insoluble in alcohol, and ether.


Glycine is widely used as a buffer for a variety of immunological applications. It is frequently used in the preparation of TG Buffers where the buffer is used as a running and/or transfer buffer for polyacrylamide gel electrophoresis and western blotting. Glycine is also used as an eluent in column chromatography. Glycine has been reported for use in the enzymatic determination of lactate using lactic dehydrogenase.


Store away from heat and oxidizing agents. Keep the container tightly closed and place it in a cool, dry and well ventilated condition. Keep it refrigerated.

Literature References

H Towbin, et al. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proc Natl Acad Sci U S A.,1979,76(9), 4350-4354.

Laemmli UK, et al. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature.,1970,227(5259), 680-5.

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