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J62534 MES, 0.5M buffer soln., pH 5.5

CAS Number

Stock No. Size Price ($) Quantity Availability
J62534-AE 100ml 51.00
J62534-AK 250ml 76.20
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MES, 0.5M buffer soln., pH 5.5


Chemical Properties

Storage & Sensitivity
Ambient temperatures.
Soluble in water (0.5 M at 20°C).


MES, is often used as a running buffer for resolving very small proteins on bis-tris gels, also used as a biological buffer in plant cell cultures. It is also been used in various protein characterization assays. This sterile solution suitable for cell culture and molecular biology applications. It is also used as a buffering agent in biology and biochemistry.


Solutions are stable at 2-8°C for months. Sterilize by filtration through 0.2 mm filters. Autoclaving is not recommended for any sulfonic acid buffer. If buffers must be nuclease-free, treat the water first, then add the buffer after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.

Literature References

BG Bugbee.; FB Salisbury. An evaluation of MES (2 (N-Morpholino) ethanesulfonic acid) and amberlite IRC-50 as pH buffers for nutrient solution studies. Journal of plant nutrition. 1985, 8, ( 7), 567-583.

MJ Alowitz.; MM Scherer . Kinetics of nitrate, nitrite, and Cr (VI) reduction by iron metal. Environmental Science & Technology. 2002, 36, (3), 299-306.

Other References

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  • J61960

    MES, 1.0M buffer soln., pH 5.0
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