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J62541 Nitrocellulose stripping buffer (10X)

CAS Number
Synonyms

Stock No. Size Price ($) Quantity Availability
J62541-AK 250ml 41.40
J62541-AP 500ml 66.10
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Nitrocellulose stripping buffer (10X)

Chemical Properties

Form
Liquid
Storage & Sensitivity
Ambient temperatures.
Solubility
Not miscible or difficult to mix in water.

Applications

Ideal for removal of secondary antibody after detection of highly abundant targets. Nitrocellulose Stripping Buffer may perform well with nitrocellulose membranes or antibody-dye conjugates. It is also employed for nucleic acid and protein related blotting works.

Notes

This buffer contains 0.5X SSC and 100 mM EDTA at pH 8.0. To strip the membrane dilute buffer to 1X in water. Warm solution to 50-70°C. Perform 2 to 3 changes (15 minutes) of the warmed 1X stripping buffer.

Literature References

Donald W. MacGlashan Jr. Endocytosis, recycling, and degradation of unoccupied FcεRI in human basophils.Journal of Leukocyte Biology.,2007,82(4), 1003-1010.

Francisco García-Carrizo, et al. Leptin Effect on Acetylation and Phosphorylation of PGC1α in Muscle Cells Associated With AMPK and Akt Activation in High-Glucose Medium.Journal of Cellular Physiology.,2015,1-30.

Other References

Harmonized Tariff Code
3822.00
TSCA
Yes

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