Cookies disclaimer

I agree Our site saves small pieces of text information (cookies) on your device in order to deliver better content and for statistical purposes. You can disable the usage of cookies by changing the settings of your browser. By browsing our website without changing the browser settings you grant us permission to store that information on your device.

Nitrocellulose stripping buffer (10X) - J62541 - Alfa Aesar

J62541 Nitrocellulose stripping buffer (10X)

CAS Number

Size Price ($) Quantity Availability
250ml 39.80
500ml 63.60
Add to Cart Add to Quote Request View Item

Nitrocellulose stripping buffer (10X)

Chemical Properties

Not miscible or difficult to mix in water.


Ideal for removal of secondary antibody after detection of highly abundant targets. Nitrocellulose Stripping Buffer may perform well with nitrocellulose membranes or antibody-dye conjugates. It is also employed for nucleic acid and protein related blotting works.


This buffer contains 0.5X SSC and 100 mM EDTA at pH 8.0. To strip the membrane dilute buffer to 1X in water. Warm solution to 50-70°C. Perform 2 to 3 changes (15 minutes) of the warmed 1X stripping buffer.

Literature References

Donald W. MacGlashan Jr. Endocytosis, recycling, and degradation of unoccupied FcεRI in human basophils.Journal of Leukocyte Biology.,2007,82(4), 1003-1010.

Francisco García-Carrizo, et al. Leptin Effect on Acetylation and Phosphorylation of PGC1α in Muscle Cells Associated With AMPK and Akt Activation in High-Glucose Medium.Journal of Cellular Physiology.,2015,1-30.

Other References

Harmonized Tariff Code


Recently Viewed


Life Science

Metals & Materials


Analytical & Labware