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Nitrocellulose stripping buffer (10X) - J62541 - Alfa Aesar

J62541 Nitrocellulose stripping buffer (10X)

CAS Number

Size Price ($) Quantity Availability
250ml 40.17
500ml 64.27
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Nitrocellulose stripping buffer (10X)

Chemical Properties

Not miscible or difficult to mix in water.


Ideal for removal of secondary antibody after detection of highly abundant targets. Nitrocellulose Stripping Buffer may perform well with nitrocellulose membranes or antibody-dye conjugates. It is also employed for nucleic acid and protein related blotting works.


This buffer contains 0.5X SSC and 100 mM EDTA at pH 8.0. To strip the membrane dilute buffer to 1X in water. Warm solution to 50-70°C. Perform 2 to 3 changes (15 minutes) of the warmed 1X stripping buffer.

Literature References

Donald W. MacGlashan Jr. Endocytosis, recycling, and degradation of unoccupied FcεRI in human basophils.Journal of Leukocyte Biology.,2007,82(4), 1003-1010.

Francisco García-Carrizo, et al. Leptin Effect on Acetylation and Phosphorylation of PGC1α in Muscle Cells Associated With AMPK and Akt Activation in High-Glucose Medium.Journal of Cellular Physiology.,2015,1-30.

Other References

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