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77-86-1 - Tris(hydroxymethyl)aminomethane, Electrophoresis Grade, 99.9% - 2-Amino-2-hydroxymethyl-1,3-propanediol - TRIS - J62569 - Alfa Aesar

J62569 Tris(hydroxymethyl)aminomethane, Electrophoresis Grade, 99.9%

CAS Number
77-86-1
Synonyms
2-Amino-2-hydroxymethyl-1,3-propanediol
TRIS

Size Price ($) Quantity Availability
100g 38.57
500g 86.56
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Tris(hydroxymethyl)aminomethane, Electrophoresis Grade, 99.9%

MDL
MFCD00004679
EINECS
201-064-4

Chemical Properties

Formula
C4H11NO3
Formula Weight
121.14
Form
Crystalline powder
Melting point
168-172°
Boiling Point
219-220°/10mm
Density
1.353
Sensitivity
Hygroscopic
Solubility
Not miscible or difficult to mix in water. Soluble in ethylene glycol (79.1 mg/ml), methanol (26 mg/ml), anhydrous ethanol (14.6 mg/ml), 95% ethanol (22 mg/ml), DMF (14 mg/ml), acetone (2 mg/ml), ethyl acetate (0.5 mg/ml), olive oil (0.4 mg/ml), chloroform (0.05 mg/ml), and alkaline solution.

Applications

A compound widely used as a biological buffer substance in the pH range 7—9; pKa = 8.3 at 20°C; pKa = 7.82 at 37°C. It is widely used as a biological buffer or as a component of buffer formulations such as TAE and TBE buffers. Tris has a pKa of 8.06 and is very useful in the biology and biochemistry lab because it has buffering capabilities in the range of the typical physiological pH of most living organisms (pH 7.0-9.0). Tris has been reported to interfere with the activity of a number of enzymes, it should therefore be used carefully when studying proteins. In medicine, Tris is used as alternative to sodium bicarbonate in the treatment of metabolic acidosis. Used in chemical manufacturing (surface-active agents, vulcanization accelerators, and pharmaceuticals), as an emulsifying agent (cosmetics, mineral oil and paraffin wax emulsions, leather dressings, textile specialties, polishes, cleaning compounds, and soluble oils), and as an absorbent for acidic gases, biological buffer, acidimetric standard, and therapeutic alkalinizing agent. Also used in buffer systems for the freezer storage of sperm, to regulate pH in the transport of live aquatic species, cell culture media, and anti-inflammatory drugs.

Notes

Store away from oxidizing agents. Keep the container tightly closed and place it in a cool, dry and well ventilated condition.

Literature References

Kallet RH, et al. The treatment of acidosis in acute lung injury with tris-hydroxymethyl aminomethane (THAM).Am J Respir Crit Care Med.,2000,161(1), 1149-53.

Desmarais WT, et al. The 1.20 A resolution crystal structure of the aminopeptidase from Aeromonas proteolytica complexed with tris: a tale of buffer inhibition.Structure.,2002,10(8), 1063-72.

GHS Hazard and Precautionary Statements

Hazard Statements: H315-H319-H335

Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.

Precautionary Statements: P261-P280-P305+P351+P338-P304+P340-P362-P312-P321-P405-P403+P233-P501a

Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. Take off contaminated clothing and wash before reuse. Call a POISON CENTER or doctor/physician if you feel unwell. Specific treatment (see label). Store locked up. Store in a well-ventilated place. Keep container tightly closed. Dispose of contents/container in accordance with local/regional/national/international regulations.

Other References

Merck
14,9772
Beilstein
741883
Harmonized Tariff Code
2922.19
TSCA
Yes
RTECS
TY2900000

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