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J62745 TE buffer, pH 8.1, RNAse free

CAS Number

Stock No. Size Price ($) Quantity Availability
J62745-=E 125ml 41.40
J62745-AK 250ml 74.50
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TE buffer, pH 8.1, RNAse free


Chemical Properties

Storage & Sensitivity
Ambient temperatures.
Not miscible or difficult to mix in water.


Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris-EDTA (TE) buffer solution, pH 8.0 may also be used as a washing buffer. TE buffer is often used to store DNA and RNA while protecting it from degradation. Some protocols use TE 10:0.1 with 0.1 mM EDTA to reduce the interaction of the EDTA with downstream applications.


200mM Tris-HCl with 20mM EDTA

For a final concentration of EDTA as 0.1 mM, dilute TE buffer 200-fold with RNase and DNase free water.

Literature References

H Ito, et al. Transformation of intact yeast cells treated with alkali cations.J. Bacteriol.,1983, 153, (1), 163-168.

Hamilton O. Smith and Max L. Birnstiel. A simple method for DNA restriction site mapping. Nucl. Acids Res.,1976, 3, (9), 2387-2398.

Other References

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