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TE buffer, pH 8.1, RNAse free - J62745 - Alfa Aesar

J62745 TE buffer, pH 8.1, RNAse free

CAS Number
Synonyms

Size Price ($) Quantity Availability
125ml 31.20
250ml 72.31
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TE buffer, pH 8.1, RNAse free

MDL
MFCD00236359

Chemical Properties

Form
Liquid
Solubility
Not miscible or difficult to mix in water.

Applications

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris-EDTA (TE) buffer solution, pH 8.0 may also be used as a washing buffer. TE buffer is often used to store DNA and RNA while protecting it from degradation. Some protocols use TE 10:0.1 with 0.1 mM EDTA to reduce the interaction of the EDTA with downstream applications.

Notes

200mM Tris-HCl with 20mM EDTA

Literature References

H Ito, et al. Transformation of intact yeast cells treated with alkali cations.J. Bacteriol.,1983,153,(1), 163-168.

Hamilton O. Smith and Max L. Birnstiel. A simple method for DNA restriction site mapping. Nucl. Acids Res.,1976,3,(9), 2387-2398.

Other References

Harmonized Tariff Code
3822.00
TSCA
Yes

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