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145224-94-8 - MES, 1.0M buffer soln., pH 7.5 - J62752 - Alfa Aesar

J62752 MES, 1.0M buffer soln., pH 7.5

CAS Number

Size Price ($) Quantity Availability
250ml 44.55
500ml 82.00
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MES, 1.0M buffer soln., pH 7.5


Chemical Properties

Soluble in water (0.5 M at 20°C).


MES, is often used as a running buffer for resolving very small proteins on bis-tris gels, also used as a biological buffer in plant cell cultures. It is also been used in various protein characterization assays. This sterile solution suitable for cell culture and molecular biology applications. It is also used as a buffering agent in biology and biochemistry.


Solutions are stable at 2-8°C for months. Sterilize by filtration through 0.2 mm filters. Autoclaving is not recommended for any sulfonic acid buffer. If buffers must be nuclease-free, treat the water first, then add the buffer after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.

Literature References

BG Bugbee.; FB Salisbury. An evaluation of MES (2 (N-Morpholino) ethanesulfonic acid) and amberlite IRC-50 as pH buffers for nutrient solution studies. Journal of plant nutrition. 1985, 8, ( 7), 567-583.

MJ Alowitz.; MM Scherer . Kinetics of nitrate, nitrite, and Cr (VI) reduction by iron metal. Environmental Science & Technology. 2002, 36, (3), 299-306.

GHS Hazard and Precautionary Statements

Hazard Statements: H315-H319-H335

Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.

Precautionary Statements: P261-P280-P305+P351+P338-P304+P340-P362-P312-P321-P405-P403+P233-P501a

Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. Take off contaminated clothing and wash before reuse. Call a POISON CENTER or doctor/physician if you feel unwell. Specific treatment (see label). Store locked up. Store in a well-ventilated place. Keep container tightly closed. Dispose of contents/container in accordance with local/regional/national/international regulations.

Other References

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