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TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. TBE Buffer is used for polyacrylamide and agarose gel electrophoresis. It is ideally suited for DNA and RNA separation on longer run, higher voltage or amperage gels. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel.5 TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Tris-Borate-EDTA buffer has been used for pulsed-field gel electrophoresis (PFGE).
Brody, J.R.; Kern, S.E. History and principles of conductive media for standard DNA electrophoresis. Anal Biochem . 2004, 333, (1), 1-13.
L Orbán and A Chrambach. Discontinuous buffer system for polyacrylamide and agarose gel electrophoresis of DNA fragments. Electrophoresis. 1991, 12, (4), 233-240.
Hazard Statements: H315-H319-H360FD
Causes skin irritation. Causes serious eye irritation. May damage fertility. Suspected of damaging the unborn child. May damage fertility. May damage the unborn child.
Precautionary Statements: P201-P202-P264b-P280i-P281-P302+P352-P305+P351+P338-P308+P313-P332+P313-P337+P313-P362-P501c
Obtain special instructions before use. Do not handle until all safety precautions have been read and understood. Wash face, hands and any exposed skin thoroughly after handling Wear eye/face protection. Use personal protective equipment as required. IF ON SKIN: Wash with plenty of soap and water. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF exposed or concerned: Get medical advice/attention. If skin irritation occurs: If eye irritation persists: Take off contaminated clothing and wash before reuse. Dispose of contents/ container to an approved waste disposal plant