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77-86-1 - TRIS-buffered saline (TBS, 10X) pH 7.4, for Western blot - J62938 - Alfa Aesar

J62938 TRIS-buffered saline (TBS, 10X) pH 7.4, for Western blot

CAS Number
77-86-1
Synonyms

Size Price ($) Quantity Availability
1L 34.10
2L 60.00
4L 113.00
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TRIS-buffered saline (TBS, 10X) pH 7.4, for Western blot

MDL
MFCD00004679

Chemical Properties

Form
Liquid
Solubility
Miscible with water.

Applications

For Western blot washing.TRIS-buffered saline (TBS, 10X) is used as a washing buffer for electrodes, alkaline phosphatase and peroxidase conjugates in dot blot assay. Further, it acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions.

Notes

500mM Tris, 1.5M NaCl, pH 7.4.Store in a cool place. Incompatible with strong oxidizing agents.

Literature References

Anastasia, A.; Barker, P. A.; Chao, M. V.; Hempstead, B. L. Detection of p75NTR Trimers: Implications for Receptor Stoichiometry and Activation. J. Neurosci. 2015, 35 (34), 11911-11920.

Brand, C. S.; Sadana, R.; Malik, S.; Smrcka, A. V.; Dessauer, C. W. Adenylyl Cyclase 5 Regulation by G βγ Involves Isoform-Specific Use of Multiple Interaction Sites. Mol. Pharmacol. 2015, 88 (4), 758-767.

Other References

Harmonized Tariff Code
2922.19
TSCA
Yes

Recommended

  • J60801

    Phosphate-buffered saline (PBS, 10X), pH 7.4, for Western blot
  • J62955

    TRIS-buffered saline (TBS, 10X), with 1% Tween 20
  • J61337

    Laemmli SDS sample buffer, reducing (6X)

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