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For Western blot washing.TRIS-buffered saline (TBS, 10X) is used as a washing buffer for electrodes, alkaline phosphatase and peroxidase conjugates in dot blot assay. Further, it acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions.
Anastasia, A.; Barker, P. A.; Chao, M. V.; Hempstead, B. L. Detection of p75NTR Trimers: Implications for Receptor Stoichiometry and Activation. J. Neurosci. 2015, 35 (34), 11911-11920.
Brand, C. S.; Sadana, R.; Malik, S.; Smrcka, A. V.; Dessauer, C. W. Adenylyl Cyclase 5 Regulation by G βγ Involves Isoform-Specific Use of Multiple Interaction Sites. Mol. Pharmacol. 2015, 88 (4), 758-767.