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HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. It is useful in the pH range 6.8-8.2. HEPES is used as a zwitterionic, all-purpose Goods buffer for use in biological research. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration when compared to bicarbonate buffers, which are also commonly used in cell culture.
David M.L. Goodgame.; Martin Joy, A. EPR study of the Cr(V) and radical species produced in the reduction of Cr(VI) by ascorbate. Inorganica Chimica Acta. 1987, 135, (2), 115-118.