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J63008 Citrate, 0.5M buffer soln., pH 2.5

CAS Number

Stock No. Size Price ($) Quantity Availability
J63008-AK 250ml 58.00
J63008-AP 500ml 91.00
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Citrate, 0.5M buffer soln., pH 2.5


Chemical Properties

Storage & Sensitivity
Keep Cold.
It is soluble in water (29.4 g/L) at 20°C. Insoluble in ethanol.


Citrate, is used as an anticoagulant and also used as a biological buffer. It is often used to prepare sodium citrate buffer for antigen retrieval of tissue samples. The citrate solution is designed to break protein cross-links, thus, unmasking antigens and epitopes in formalin-fixed and paraffin embedded tissue sections, resulting in enhancing staining intensity of antibodies. Citrate has anticoagulant activity and as a calcium chelator, it forms complexes that disrupt the tendency of blood to clot.


Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Keep away from strong oxidizing agents.

Literature References

YP Lee.; T Takahashi. An improved colorimetric determination of amino acids with the use of ninhydrin. Analytical Biochemistry. 1972, 14,(1),  71-77.

XZ Shu.; KJ Zhu.; W Song. Novel pH-sensitive citrate cross-linked chitosan film for drug controlled release. International Journal of Pharmaceutics. 2001, 212,(1), 19-28.

Other References

Harmonized Tariff Code


  • 42423

    Buffer solution, pH 9.54 (±0.01 @ 25°C), No Color, Specpure®, NIST Traceable
  • 44080

    Poly(vinylidene fluoride)
  • J62131

    TRIS, 0.5M buffer soln., pH 8.5
  • J62261

    MOPS, 1.0M buffer soln., pH 5.5
  • J62446

    CAPS, 0.5M buffer soln., pH 10.5

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