Denhardt's solution (50X)
Storage & Sensitivity
Store at -20°C.
Denhardt's solution is used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. Denhardt's Solution is a mixture of blocking agents used in membrane-based hybridization protocols.
1% Ficolll (Type 400, Pharmacia), 1% polyvinylpyrrolidone, and 1% bovine serum albumin