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Sodium citrate, 0.5M buffer soln., pH 5.5 - J63199 - Alfa Aesar

J63199 Sodium citrate, 0.5M buffer soln., pH 5.5

CAS Number
Synonyms

Size Price ($) Quantity Availability
250ml 48.20
500ml 72.31
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Sodium citrate, 0.5M buffer soln., pH 5.5

MDL
MFCD00012462

Chemical Properties

Form
Liquid
Solubility
Soluble in water.

Applications

Sodium citrate is commonly used in nucleic acid hybridization techniques at concentrations ranging from 0.1X to 20X depending on the application.  It is used to facilitate transfer of nucleic acids to membranes in southern and northern blotting, dot blotting, and colony and plaque lifts. 

Notes

20X SSC Solution (RNase-free) should be stored at room temperature. This reagent should remain stable for at least 1 year in its unopened container.

Literature References

CJ Quaife.; SD Findley.; JC Erickson.; GJ Froelick. Induction of a new metallothionein isoform (MT-IV) occurs during differentiation of stratified squamous epithelia. Biochemistry. 1994, 33,(23), 7250-7259.

M Cappello.; S Keshav.; C Prince.; DP Jewell.; S Gordon. Detection of mRNAs for macrophage products in inflammatory bowel disease by in situ hybridization. Gut . 1992, 33,(9), 1214-1219.

GHS Hazard and Precautionary Statements

Hazard Statements: H319

Causes serious eye irritation.

Precautionary Statements: P280-P264-P305+P351+P338-P337+P313

Wear protective gloves/protective clothing/eye protection/face protection. Wash thoroughly after handling. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice/attention.

Other References

Harmonized Tariff Code
2918.15
TSCA
No

Recommended

  • J60763

    MES, 1.0M buffer soln., pH 6.0
  • J62918

    Sodium citrate, 0.5M buffer soln., pH 5.0

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