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J63314 Tween 20 washing buffer, 1% in PBS (10X)

CAS Number
Synonyms

Stock No. Size Price ($) Quantity Availability
J63314-AP 500ml 40.20
J63314-K2 1L 59.50
J63314-K3 2L 112.00
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Tween 20 washing buffer, 1% in PBS (10X)

Chemical Properties

Form
Viscous liquid
Storage & Sensitivity
Keep Cold.
Solubility
Difficult to mix.

Applications

Tween-20 is a non-ionic surfactant that is useful for solubilizing membrane proteins during isolation. Tween-20 is also added to buffers for immunohistochemistry. Tween-20 may also help to prevent non-specific antibody binding and function as a blocking agent in immunoblotting assays. It has been used in preextraction of membranes to remove peripheral proteins.

Notes

Keep container tightly closed. Store in cool, dry conditions in well sealed containers.

Literature References

Nicole Schaeren-Wiemers.; Andrea Gerfin-Moser. A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: in situ hybridization using digoxigenin-labelled cRNA probes. Histochemistry.1993 , 100, (6), 431-440.

Eric Lecuyer.; Neela Parthasarathy.; Henry M. Krause. Fluorescent In Situ Hybridization Protocols in Drosophila Embryos and Tissues. Drosophila.2008, 420, 289-302.

Other References

Harmonized Tariff Code
3822.00
TSCA
Yes

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