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J63314 Tween 20 washing buffer, 1% in PBS (10X)

CAS Number

Stock No. Size Price ($) Quantity Availability
J63314-AP 500ml 41.40
J63314-K2 1L 61.30
J63314-K3 2L 115.00
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Tween 20 washing buffer, 1% in PBS (10X)

Chemical Properties

Viscous liquid
Storage & Sensitivity
Keep Cold.
Difficult to mix.


Tween-20 is a non-ionic surfactant that is useful for solubilizing membrane proteins during isolation. Tween-20 is also added to buffers for immunohistochemistry. Tween-20 may also help to prevent non-specific antibody binding and function as a blocking agent in immunoblotting assays. It has been used in preextraction of membranes to remove peripheral proteins.


Keep container tightly closed. Store in cool, dry conditions in well sealed containers.

Literature References

Nicole Schaeren-Wiemers.; Andrea Gerfin-Moser. A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: in situ hybridization using digoxigenin-labelled cRNA probes. Histochemistry.1993 , 100, (6), 431-440.

Eric Lecuyer.; Neela Parthasarathy.; Henry M. Krause. Fluorescent In Situ Hybridization Protocols in Drosophila Embryos and Tissues. Drosophila.2008, 420, 289-302.

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