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Imidazole is used as a chelator for the binding of various divalent cations, and is also used to elute tagged proteins bound to Ni ions attached to the surface of beads in the chromatography column.
Chihuey Wong.; Dale G. Drueckhammer.; Henri M. Sweers. Enzymatic vs. fermentative synthesis: thermostable glucose dehydrogenase catalyzed regeneration of NAD(P)H for use in enzymatic synthesis.J. Am. Chem. Soc.1985, (13), 4028-4031.
Hazard Statements: H315-H318-H360D
Causes skin irritation. Causes serious eye damage. May damage the unborn child.
Precautionary Statements: P201-P202-P264b-P281-P302+P352-P305+P351+P338-P308+P313-P310-P332+P313-P362-P501c
Obtain special instructions before use. Do not handle until all safety precautions have been read and understood. Wash face, hands and any exposed skin thoroughly after handling Use personal protective equipment as required. IF ON SKIN: Wash with plenty of soap and water. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF exposed or concerned: Get medical advice/attention. Immediately call a POISON CENTER or doctor/physician. If skin irritation occurs: Take off contaminated clothing and wash before reuse. Dispose of contents/ container to an approved waste disposal plant