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Tween 20 washing buffer, 0.5% in PBS (10X) is used as a wash solution for western blot membranes and microtiter plate wells in ELISA assays. It acts as a pH stabilizer in optimal formulation, salts and detergents designed to effectively remove excess material from membranes. It finds application as laboratory detergents as well as in biomedical experiments.
Kaur, M.; Malik, B.; Garg, T.; Rath, G.; Goyal, A. K. Development and characterization of guar gum nanoparticles for oral immunization against tuberculosis. Drug delivery 2015, 22 (3), 328-334.
Soler, M.; Estevez, M. C.; Alvarez, M.; Otte, M. A.; Sepulveda, B.; Lechuga, L. M. Direct Detection of Protein Biomarkers in Human Fluids Using Site-Specific Antibody Immobilization Strategies. Sensors 2014, 14 (2), 2239-2258.