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39450-01-6 - Proteinase K, Ready-to-Use soln. - J63710 - Alfa Aesar

J63710 Proteinase K, Ready-to-Use soln.

CAS Number
39450-01-6
Synonyms

Size Price ($) Quantity Availability
5ml 244.11
25ml 748.80
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Proteinase K, Ready-to-Use soln.

MDL
MFCD00132129
EINECS
254-457-8

Chemical Properties

Form
Liquid, 28.9 kDa
Solubility
Miscible with water.

Applications

A stable and highly reactive serine protease; used for protein and nucleic acid isolation.Proteinase K is used in the purification of RNA and DNA from tissues or cell lines. It finds application in protein modification, determination of enzyme localization and inactivation of RNases/DNases during nucleic acid extraction. It is also used in mitochondria isolation and removal of endotoxins bound to cationic proteins like lysozyme and ribonuclease A. Further, it is involved in the treatment of paraffin embedded tissue sections in order to expose antigen binding sites for antibody labeling.

Notes

Minimum 400 units per ml. Protein concentration is 20 mg/ml in 10mM Tris-HCl, 1mM calcium acetate, pH 7.5 containing 50% glycerol. RNase and DNase free. One unit releases one micromole of Folin positive amino acids, measured as tyrosine, at 37°C and pH 7.5, using urea denatured hemoglobin as the substrate.Incompatible with strong oxidizing agents. Store in a cool place.

Literature References

Fränzel, B.; Fischer, F.; Steegborn, C.; Wolters, D. A. Proteinase K improves quantitative acylation studies. Proteomics 2015, 15 (1), 44-47.

Shishova, K. V.; Khodarovich, Y. M.; Lavrentyeva, E. A.; Zatsepina, O. V. Analysis of the localization of fibrillarin and sites of pre-rRNA synthesis in the nucleolus-like bodies of mouse GV oocytes after mild treatment with proteinase K. Russ. J. Dev. Biol. 2015, 46 (3), 127-136.

GHS Hazard and Precautionary Statements

Hazard Statements: H334-H319

May cause allergy or asthma symptoms or breathing difficulties if inhaled. Causes serious eye irritation.

Precautionary Statements: P285-P261-P280-P264-P305+P351+P338-P342+P311-P304+P341-P337+P313-P501a

In case of inadequate ventilation wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. Wash thoroughly after handling. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician. IF INHALED: If breathing is difficult, remove to fresh air and keep at rest in a position comfortable for breathing. If eye irritation persists: Get medical advice/attention. Dispose of contents/container in accordance with local/regional/national/international regulations.

Other References

Harmonized Tariff Code
3507.90
TSCA
No

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