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BSA blocking buffer, 5% in PBS, with 0.05% Tween-20 - J63711 - Alfa Aesar

J63711 BSA blocking buffer, 5% in PBS, with 0.05% Tween-20

CAS Number

Size Price ($) Quantity Availability
250ml 71.60
500ml 120.00
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BSA blocking buffer, 5% in PBS, with 0.05% Tween-20

Chemical Properties

Insoluble in water.


Buffered BSA solution used to block ELISA strips and as a diluent of primary and secondary antibodies used for ELISA applications. BSA is useful for saturating excess protein-binding sites on membranes and microplates in immunoassays. Blocker BSA is usually more effective than nonfat milk blocking buffers for biotin-avidin systems. Use as a diluent for antigens, antibodies, and other proteins only after sensitization of the microwell plate or membrane.


5% Bovine serum albumin in phosphate-buffered saline, 0.05% Tween-20, pH 7.4

Literature References

Costas M. Pitsillides,; Edwin K. Joe,; Xunbin Wei,; R. Rox Anderson,; Charles P. Lin.Selective Cell Targeting with Light-Absorbing Microparticles and Nanoparticles. Biophysical journal. 2003 , 84 (6), 4023-4032.

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