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Buffered BSA solution used to block ELISA strips and as a diluent of primary and secondary antibodies used for ELISA applications. BSA is useful for saturating excess protein-binding sites on membranes and microplates in immunoassays. Blocker BSA is usually more effective than nonfat milk blocking buffers for biotin-avidin systems. Use as a diluent for antigens, antibodies, and other proteins only after sensitization of the microwell plate or membrane.
Costas M. Pitsillides,; Edwin K. Joe,; Xunbin Wei,; R. Rox Anderson,; Charles P. Lin.Selective Cell Targeting with Light-Absorbing Microparticles and Nanoparticles. Biophysical journal. 2003 , 84 (6), 4023-4032.