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J63711 BSA blocking buffer, 5% in PBS, with 0.05% Tween-20

CAS Number
Synonyms

Stock No. Size Price ($) Quantity Availability
J63711-AK 250ml 74.50
J63711-AP 500ml 125.00
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BSA blocking buffer, 5% in PBS, with 0.05% Tween-20

Chemical Properties

Form
Liquid
Storage & Sensitivity
Store at -20°C.
Solubility
Insoluble in water.

Applications

Buffered BSA solution used to block ELISA strips and as a diluent of primary and secondary antibodies used for ELISA applications. BSA is useful for saturating excess protein-binding sites on membranes and microplates in immunoassays. Blocker BSA is usually more effective than nonfat milk blocking buffers for biotin-avidin systems. Use as a diluent for antigens, antibodies, and other proteins only after sensitization of the microwell plate or membrane.

Notes

5% Bovine serum albumin in phosphate-buffered saline, 0.05% Tween-20, pH 7.4

Literature References

Costas M. Pitsillides,; Edwin K. Joe,; Xunbin Wei,; R. Rox Anderson,; Charles P. Lin.Selective Cell Targeting with Light-Absorbing Microparticles and Nanoparticles. Biophysical journal. 2003 , 84 (6), 4023-4032.

Other References

Harmonized Tariff Code
3822.00
TSCA
Yes

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