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Sodium citrate, 0.5M buffer soln., pH 6.5 - J63888 - Alfa Aesar

J63888 Sodium citrate, 0.5M buffer soln., pH 6.5

CAS Number

Size Price ($) Quantity Availability
250ml 48.20
500ml 72.31
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Sodium citrate, 0.5M buffer soln., pH 6.5


Chemical Properties

Soluble in water.


Sodium citrate is commonly used in nucleic acid hybridization techniques at concentrations ranging from 0.1X to 20X depending on the application.  It is used to facilitate transfer of nucleic acids to membranes in southern and northern blotting, dot blotting, and colony and plaque lifts. 


20X SSC Solution (RNase-free) should be stored at room temperature. This reagent should remain stable for at least 1 year in its unopened container.

Literature References

CJ Quaife.; SD Findley.; JC Erickson.; GJ Froelick. Induction of a new metallothionein isoform (MT-IV) occurs during differentiation of stratified squamous epithelia. Biochemistry. 1994, 33,(23), 7250-7259.

M Cappello.; S Keshav.; C Prince.; DP Jewell.; S Gordon. Detection of mRNAs for macrophage products in inflammatory bowel disease by in situ hybridization. Gut . 1992, 33,(9), 1214-1219.

Other References

Harmonized Tariff Code


  • 12325

    Hydrogen tetrachloroaurate(III) hydrate, 99.9% (metals basis), Au 49% min
  • 38712

    Buffer solution, pH 7.00 (±0.01 @ 25°C), No Color, Specpure®, NIST Traceable
  • J61141

    TRIS, 0.5M buffer soln., pH 7.5
  • J62131

    TRIS, 0.5M buffer soln., pH 8.5
  • J62688

    HEPES, 1.0M buffer soln., pH 7.0

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