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TAE (50X), TRIS + acetate + EDTA is used in electrophoresis of nucleic acids in agarose and polyacrylamide gel. It acts as a running buffer and as a gel preparation buffer. It is also useful for nucleic acid separations viz. DNA and RNA. Further, it is used for non-denaturing RNA agarose gel electrophoresis.
Lechanteur, A.; Furst, T.; Evrard, B.; Delvenne, P.; Hubert, P.; Piel, G. Development of anti-E6 pegylated lipoplexes for mucosal application in the context of cervical preneoplastic lesions. Int. J. Pharm. 2015, 483 (1-2), 268-277.
Kaur, K.; Kumar, V.; Beniwal, V.; Kumar, V.; Aneja, K. R.; Sharma, V.; Jaglan, S. T. Solvent-free synthesis of novel (E)-2-(3, 5-dimethyl-4-(aryldiazenyl)-1H-pyrazol-1-yl)-4-arylthiazoles: determination of their biological activity. Med. Chem. Res. 2015, 24 (11), 3863-3875.
Hazard Statements: H315-H319-H335
Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.
Precautionary Statements: P261-P264b-P271-P280-P302+P352-P304+P340-P305+P351+P338-P312-P332+P313-P337+P313-P362-P501c
Avoid breathing dust/fume/gas/mist/vapours/spray. Wash face, hands and any exposed skin thoroughly after handling Use only outdoors or in a well-ventilated area. Wear protective gloves/protective clothing/eye protection/face protection. IF ON SKIN: Wash with plenty of soap and water. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Call a POISON CENTER or doctor/physician if you feel unwell. If skin irritation occurs: Get medical advice/attention. If eye irritation persists: Take off contaminated clothing and wash before reuse. Dispose of contents/ container to an approved waste disposal plant