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9002-07-7 - Trypsin 1:250, porcine pancreas - EC 3.4.21.4 - J63993 - Alfa Aesar

J63993 Trypsin 1:250, porcine pancreas

CAS Number
9002-07-7
Synonyms
EC 3.4.21.4

Size Price ($) Quantity Availability
10g 123.00
50g 210.00
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Trypsin 1:250, porcine pancreas

MDL
MFCD01775455
EINECS
232-650-8

Chemical Properties

Form
Powder
Solubility
Soluble in Hank's Balanced Salt Solution (35 mg/ml), HCl (1 mM), and water. Insoluble in clycerol and alcohol.

Applications

Trypsin 1:250 is used in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. It has also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestions. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.

Notes

Low endotoxin. Minimum 225 units/mg. One unit causes a decrease in absorbance at 253 nm of 0.003 per minute at 25C.

Literature References

Paul P.Geurink; Wouter A van der Linden; Anne C.Mirabella; Nerea Gallastegui; Gerjan de Bruin; Annet E.M.Blom; Mathias J.Voges; Elliot D.Mock; Bogdan I.Florea; Gijs A van der Marel; Christoph Driessen; Mario van der Stelt; Michael Groll; Herman S.Overkleeft; Alexei F.Kisselev. Incorporation of non-natural amino acids improves cell permeability and potency of specific inhibitors of proteasome trypsin-like sites. Journal of medicinal and pharmaceutical chemistry. 2013, 56,(3), 1262-1275.

 

Simone A.Beckham; Jason Brouwer; Anna Roth; Die Wang; Anthony J.Sadler; Matthias John; Kerstin Jahn-Hofmann; Bryan R.G.Williams; Jacqueline A.Wilce; Matthew C.J.Wilce. Conformational rearrangements of RIG-I receptor on formation of a multiprotein:dsRNA assembly. Nucleic Acids Research. 2013, 41,(5), 3436-3445.

GHS Hazard and Precautionary Statements

Hazard Statements: H315-H319-H334-H335

Causes skin irritation. Causes serious eye irritation. May cause allergy or asthma symptoms or breathing difficulties if inhaled. May cause respiratory irritation.

Precautionary Statements: P284-P261-P280a-P305+P351+P338-P405-P501a

Wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves and eye/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Other References

Merck
14,9795
Harmonized Tariff Code
3507.90
TSCA
Yes
RTECS
YN5075000

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