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J63993 Trypsin 1:250, porcine pancreas

CAS Number
9002-07-7
Synonyms
EC 3.4.21.4

Stock No. Size Price ($) Quantity Availability
J63993-09 10g 130.00
J63993-18 50g 218.00
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Trypsin 1:250, porcine pancreas

MDL
MFCD01775455
EINECS
232-650-8

Chemical Properties

Form
Powder
Storage & Sensitivity
Store at -20°C.
Solubility
Soluble in Hank's Balanced Salt Solution (35 mg/ml), HCl (1 mM), and water. Insoluble in clycerol and alcohol.

Applications

Trypsin 1:250 is used in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. It has also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestions. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.

Notes

Low endotoxin. Minimum 225 units/mg. One unit causes a decrease in absorbance at 253 nm of 0.003 per minute at 25C.

Literature References

Paul P.Geurink; Wouter A van der Linden; Anne C.Mirabella; Nerea Gallastegui; Gerjan de Bruin; Annet E.M.Blom; Mathias J.Voges; Elliot D.Mock; Bogdan I.Florea; Gijs A van der Marel; Christoph Driessen; Mario van der Stelt; Michael Groll; Herman S.Overkleeft; Alexei F.Kisselev. Incorporation of non-natural amino acids improves cell permeability and potency of specific inhibitors of proteasome trypsin-like sites. Journal of medicinal and pharmaceutical chemistry. 2013, 56,(3), 1262-1275.

 

Simone A.Beckham; Jason Brouwer; Anna Roth; Die Wang; Anthony J.Sadler; Matthias John; Kerstin Jahn-Hofmann; Bryan R.G.Williams; Jacqueline A.Wilce; Matthew C.J.Wilce. Conformational rearrangements of RIG-I receptor on formation of a multiprotein:dsRNA assembly. Nucleic Acids Research. 2013, 41,(5), 3436-3445.

GHS Hazard and Precautionary Statements

Hazard Statements: H315-H319-H334-H335

Causes skin irritation. Causes serious eye irritation. May cause allergy or asthma symptoms or breathing difficulties if inhaled. May cause respiratory irritation.

Precautionary Statements: P261-P264b-P271-P280-P285-P302+P352-P304+P340-P305+P351+P338-P332+P313-P337+P313-P342+P311-P362-P501c

Avoid breathing dust/fume/gas/mist/vapours/spray. Wash face, hands and any exposed skin thoroughly after handling Use only outdoors or in a well-ventilated area. Wear protective gloves/protective clothing/eye protection/face protection. In case of inadequate ventilation wear respiratory protection. IF ON SKIN: Wash with plenty of soap and water. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If skin irritation occurs: Get medical advice/attention. If eye irritation persists: If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician. Take off contaminated clothing and wash before reuse. Dispose of contents/ container to an approved waste disposal plant

Other References

Merck
14,9795
Harmonized Tariff Code
3507.90
TSCA
Yes
RTECS
YN5075000

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