Labels both vascular endothelial cells and macrophages. It can be used to identify and/or isolate these cells from mixed cell populations. When cells are labeled with DiO-Ac-LDL, the lipoprotein is degraded by lysosomal enzymes and the fluorescent probe accumulates in the intracellular membranes. Labeling cells with DiO-Ac-LDL has no effect on cell viability. Pure cultures of vascular endothelial cells can be isolated from complex primary cultures using fluorescent activated cell sorting.DiO-Lipoprotein, low density, acetylated, human plasma is useful for fixed wavelength fluorescence activated cell sorting (FACS) Cell sorters.
Ferri, N.; Tibolla, G.; Pirillo, A.; Cipollone, F.; Mezzetti, A.; Pacia, S.; Corsini, A.; Catapano, A. L. Proprotein convertase subtilisin kexin type 9 (PCSK9) secreted by cultured smooth muscle cells reduces macrophages LDLR levels. Atherosclerosis 2012, 220 (2), 381-386.
Okura, H.; Saga, A.; Fumimoto, Y.; Soeda, M.; Moriyama, M.; Moriyama, H.; Nagai, K.; Lee, C. M.; Yamashita, S.; Ichinose, A.; Hayakawa, T.; Matsuyama, A. Transplantation of Human Adipose Tissue-Derived Multilineage Progenitor Cells Reduces Serum Cholesterol in Hyperlipidemic Watanabe Rabbits. Tissue Eng. Part C Methods 2011, 17 (2), 145-154.