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6104-59-2 - Brilliant Blue R - Coomassie Brilliant Blue R 250 - C.I. 42660 - J64297 - Alfa Aesar

J64297 Brilliant Blue R

CAS Number
6104-59-2
Synonyms
Coomassie Brilliant Blue R 250
C.I. 42660

Size Price ($) Quantity Availability
50g 72.82
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Brilliant Blue R

MDL
MFCD00041762
EINECS
228-060-5

Chemical Properties

Formula
C45H44N3NaO7S2
Formula Weight
825.97
Form
Crystalline powder
Solubility
Soluble in water.

Applications

Brilliant Blue R is used for detection of protein bands following electrophoresis. It is a sensitive stain for protein detection in polyacrylamide gels, typically giving blue bands on a clear background with a sensitivity of 50-100 ng/band. It may be combined with other stains, such as silver stain, to distinguish different types of proteins. It displays metachromasia by appearing pink-red, rather than blue, when binding certain proteins, such as collagen and histone H1.

Notes

Stable under recommended storage conditions. Incompatible with oxidizing agents.

Literature References

James Dzandu; M.E.Deh; D.L.Barratt; G.E.Wise. Detection of erythrocyte membrane proteins, sialoglycoproteins, and lipids in the same poly-acrylamide gel using a double-staining technique. Proceedings of the National Academy of Sciences. 1984, 81,(6), 1733-1737.

 

H.Chen; H.Cheng; M.Bjerknes. One-step Coomassie brilliant blue R-250 staining of proteins in polyacrylamide gel. Analytical Biochemistry. 1993, 212,(1), 295-296.

Other References

Beilstein
5718025
Harmonized Tariff Code
3204.12
TSCA
Yes

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