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Ammonium sulfate is a widely used reagent in molecular biology and chromatography. It is useful in the isolation and purification of enzymes and proteins. It is used for the precipitation or fractionation of proteins or for purification of antibodies. It is also useful for crystallographic analysis of nucleic acids and proteins. Ammonium sulfate is also widely used in HPLC of proteins, such as in hydrophobic interaction chromatography. Ammonium sulfate is used in long PCR buffer, in PCR lysis solution, and in secondstrand cDNA synthesis. Also it is used for the HPLC analysis of phospholipids.
Gilad Haran.; Rivka Cohen.; Liliana K Bar.; Yechezkel Barenholz. Transmembrane ammonium sulfate gradients in liposomes produce efficient and stable entrapment of amphipathic weak bases. Biochimica et Biophysica Acta (BBA) - Biomembranes. 1993, 1151 (2), 201-215.
Tage Astrup.; Sten Mullertz. The fibrin plate method for estimating fibrinolytic activity. Archives of Biochemistry and Biophysics. 1952, 40 (2), 346-351.
Hazard Statements: H303
May be harmful if swallowed.
Precautionary Statements: P312a
Call a POISON CENTER/doctor if you feel unwell.