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Z-Asp-Glu-Val-Asp-7-amino-4-(trifluoromethyl)coumarin acts as a substrate for granzyme B-processed ICE-LAP3, caspase-6, caspase-7, caspase-8, and caspase-10. The reaction can be observed by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.
Jiuru Sun.; Stephen P. Bottomley.; Sharad Kumar.; Phillip I. Bird. Recombinant Caspase-3 Expressed inPichia pastorisIs Fully Activated and Kinetically Indistinguishable from the Native Enzyme. Biochemical and Biophysical Research Communications. 1997, 238 (3), 920-924.
Kieran F. Harvey.; Natasha L. Harvey.; Julie M. Michael.; Gayathri Parasivam.; Nigel Waterhouse.; Emad S. Alnemri?.; Dianne Watters.; Sharad Kumar. Caspase-mediated Cleavage of the Ubiquitin-protein Ligase Nedd4 during Apoptosis. The Journal of Biological Chemistry. 1998, 273 13524-13530.