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J65104 Guanidine thiocyanate, Molecular Biology Grade

CAS Number
593-84-0
Synonyms
Guanidine rhodanide

Stock No. Size Price ($) Quantity Availability
J65104-18 50g 80.20
J65104-30 250g 166.00
J65104-36 500g 280.00
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Guanidine thiocyanate, Molecular Biology Grade

MDL
MFCD00013027
EINECS
209-812-1

Chemical Properties

Formula
CH5N3.CHNS
Formula Weight
118.16
Form
Crystalline solid
Melting point
118-122°
Density
1.29
Storage & Sensitivity
Light Sensitive. Ambient temperatures.
Solubility
Soluble in water (1420 g/L) at 20°C, and ethanol (5%).

Applications

Guanidine thiocyanate is used for RNA isolation and can solubilize proteins. When the compound is used to solubilize proteins it can be removed by dialysis against urea without difficulty. The hybridization of guanidine thiocyanate-solubilize hepatocytes can be used as a method to quantify mRNA from hepatocytes. Blood sample lysate stored in guanidine thiocyanate has been shown to maintain nucleic acid integrity. Research describes guanidine thiocyanate to be ineffective for RNA isolation of plant tissue with a high levels of secondary metabolites and storage proteins.

Notes

Light Sensitive. Keep container tightly closed. Store away from oxidizing agents.

Literature References

Gregory J. Wadsworth.; Margaret G. Redinbaugh.; John G. Scandalios. A procedure for the small-scale isolation of plant RNA suitable for RNA blot analysis . Analytical Biochemistry. 1988, 172, (1), 279-283.

John M. Chirgwin.; Alan E. Przybyla.; Raymond J. MacDonald.; William J. Rutter. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979, 18, (24), 5294-5299.

GHS Hazard and Precautionary Statements

Hazard Statements: H302+H312+H332-H314

Harmful if swallowed. Harmful in contact with skin. Harmful if inhaled. Causes severe skin burns and eye damage.

Precautionary Statements: P260-P264b-P270-P271-P280-P284-P303+P361+P353-P304+P340-P305+P351+P338-P310-P330-P331-P363-P501c

Do not breathe dust/fume/gas/mist/vapours/spray. Wash face, hands and any exposed skin thoroughly after handling Do not eat, drink or smoke when using this product. Use only outdoors or in a well-ventilated area. Wear protective gloves/protective clothing/eye protection/face protection. Wear respiratory protection. IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician. Rinse mouth. Do NOT induce vomiting. Wash contaminated clothing before reuse. Dispose of contents/ container to an approved waste disposal plant

Other References

Beilstein
3563461
Hazard Class
8
Packing Group
III
Harmonized Tariff Code
2925.29
TSCA
Yes
RTECS
XL1225000

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