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9004-07-3 - alpha-Chymotrypsin, from bovine pancreas - EC 3.4.21.1 - J65266 - Alfa Aesar

J65266 alpha-Chymotrypsin, from bovine pancreas

CAS Number
9004-07-3
Synonyms
EC 3.4.21.1

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alpha-Chymotrypsin, from bovine pancreas

MDL
MFCD00130481
EINECS
232-671-2

Chemical Properties

Formula Weight
25kDa
Form
Lyophilized powder
Solubility
Reconstitute in 1mM HCl. Soluble at 10mg/ml in 1mM HCl. 2mM calcium chloride serves as a stabilizer. Store aliquoted solutions at -20°C for up to a week.

Applications

α-Chymotrypsin from bovine pancreas is used for protein identification by mass spectrometry, the isolation and characterization of myosin heavy chains, the incubation of infected and uninfected cells for analysis of cellular proteins by SDS-PAGE. A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

Notes

Suitable for protein sequencing. 3X crystallized and treated with TLCK to inhibit trypsin. Dialyzed to remove autolysis products and low molecular weight contaminants. One unit with hydrolyze one micromole of benzoyl-L-tyrosine ethyl ester per minute at 25°C, pH 7.8.

Literature References

Tessa E.F. Quax; Mart Krupovic; Soizick Lucas; Patrick Forterre; David Prangishvili; Tessa E.F. Quax; Mart Krupovic; Soizick Lucas; Patrick Forterre; David Prangishvili. Structure of the Bovine Antimicrobial Peptide Indolicidin Bound to Dodecylphosphocholine and Sodium Dodecyl Sulfate Micelles. Virology. 2010, 404, (1), 1-4.

Miguel Ribeiro; Emmanuelle Bancel; Annie Faye; Mireille Dardevet; Catherine Ravel; Gerard Branlard; Gilberto Igrejas. Proteogenomic Characterization of Novel x-Type High Molecular Weight Glutenin Subunit 1Ax1.1. International Journal of Molecular Sciences. 2013, 14, (3), 5650-5667.

GHS Hazard and Precautionary Statements

Hazard Statements: H315-H319-H334-H335

Causes skin irritation. Causes serious eye irritation. May cause allergy or asthma symptoms or breathing difficulties if inhaled. May cause respiratory irritation.

Precautionary Statements: P284-P261-P280a-P305+P351+P338-P405-P501a

Wear respiratory protection. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves and eye/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Other References

Harmonized Tariff Code
3507.90
TSCA
Yes
RTECS
GC3050000

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