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ANS forms an inclusion complex with cyclodextrin. Such model systems are useful to mimic biological recognition and can be studied by measuring the change in fluorescence of free-ANS to complexed-ANS. When ANS enters the hydrophobic core of cyclodestrin, its fluorescence increases. It is commonly used to study molecular assemblies of surfactants and amphiphilic polymers because a blue shift of the emission maximum indicates the fluorophore is located in less polar media.
Neha Jain.; Mily Bhattacharya.; Samrat Mukhopadhyay. Kinetics of Surfactant-induced Aggregation of Lysozyme Studied by Fluorescence Spectroscopy. Journal of Fluorescence. 2011, 21, (2), 615-625.