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DiI-Ac-LDL is used to label vascular endothelial cells and macrophages and isolate these cells from mixed cell populations. When cells are labeled with DiI-Ac-LDL, the lipoprotein is degraded by lysosomal enzymes and the fluorescent probe accumulates in the intracellular membranes.DiI-Lipoprotein, low density, acetylated, human plasma is used to flag both macrophages and vascular endothelial cells in order to identify and isolate these from populations of mixed cells.
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