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Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.
Dipti Arora.; Navin Khanna. Method for increasing the yield of properly folded recombinant human gamma interferon from inclusion bodies. Journal of Biotechnology. 1996, 52, (2), 127-133.
Cing-Yuen Won.; Maurice R. Eftin. Incorporation of Tryptophan Analogues into Staphylococcal Nuclease, Its V66W Mutant, and Δ137-149 Fragment:? Spectroscopic Studies. Biochemistry. 1998, 37, (25), 8938-8946.
Hazard Statements: H302-H315-H319-H332-H500
Harmful if swallowed. Causes skin irritation. Causes serious eye irritation. Harmful if inhaled. May form combustible dust concentrations in air
Precautionary Statements: P261-P264b-P270-P271-P280-P301+P312-P302+P352-P304+P340-P305+P351+P338-P312-P330-P332+P313-P337+P313-P362-P501c
Avoid breathing dust/fume/gas/mist/vapours/spray. Wash face, hands and any exposed skin thoroughly after handling Do not eat, drink or smoke when using this product. Use only outdoors or in a well-ventilated area. Wear protective gloves/protective clothing/eye protection/face protection. IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell. IF ON SKIN: Wash with plenty of soap and water. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Rinse mouth. If skin irritation occurs: Get medical advice/attention. If eye irritation persists: Take off contaminated clothing and wash before reuse. Dispose of contents/ container to an approved waste disposal plant