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50-01-1 - Guanidine hydrochloride, Molecular Biology Grade - J65661 - Alfa Aesar

J65661 Guanidine hydrochloride, Molecular Biology Grade

CAS Number
50-01-1
Synonyms

Size Price ($) Quantity Availability
100g 85.00
500g 297.00
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Guanidine hydrochloride, Molecular Biology Grade

MDL
MFCD00013026
EINECS
200-002-3

Chemical Properties

Formula
CH5N3•HCl
Formula Weight
95.53
Form
Crystalline solid
Melting point
178-185°
Density
1.34
Sensitivity
Hygroscopic
Solubility
Freely soluble in water

Applications

Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.

Notes

Protect from heat. Store away from oxidizing agents.

Literature References

Dipti Arora.; Navin Khanna. Method for increasing the yield of properly folded recombinant human gamma interferon from inclusion bodies. Journal of Biotechnology. 1996, 52, (2), 127-133.

Cing-Yuen Won.; Maurice R. Eftin. Incorporation of Tryptophan Analogues into Staphylococcal Nuclease, Its V66W Mutant, and Δ137-149 Fragment:? Spectroscopic Studies. Biochemistry. 1998, 37, (25), 8938-8946.

GHS Hazard and Precautionary Statements

Hazard Statements: H302-H315-H319

Harmful if swallowed. Causes skin irritation. Causes serious eye irritation.

Precautionary Statements: P280-P264-P270-P305+P351+P338-P362-P301+P312-P321-P332+P313-P337+P313-P501a

Wear protective gloves/protective clothing/eye protection/face protection. Wash thoroughly after handling. Do not eat, drink or smoke when using this product. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Take off contaminated clothing and wash before reuse. IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell. Specific treatment (see label). If skin irritation occurs: Get medical advice/attention. If eye irritation persists: Dispose of contents/container in accordance with local/regional/national/international regulations.

Other References

Merck
14,4562
Beilstein
3591990
Harmonized Tariff Code
2925.29
TSCA
Yes
RTECS
MF4300000

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