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J65661 Guanidine hydrochloride, Molecular Biology Grade

CAS Number
50-01-1
Synonyms

Stock No. Size Price ($) Quantity Availability
J65661-22 100g 87.20
J65661-36 500g 303.00
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Guanidine hydrochloride, Molecular Biology Grade

MDL
MFCD00013026
EINECS
200-002-3

Chemical Properties

Formula
CH5N3•HCl
Formula Weight
95.53
Form
Crystalline solid
Melting point
178-185°
Density
1.34
Storage & Sensitivity
Hygroscopic. Ambient temperatures.
Solubility
Freely soluble in water

Applications

Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.

Notes

Protect from heat. Store away from oxidizing agents.

Literature References

Dipti Arora.; Navin Khanna. Method for increasing the yield of properly folded recombinant human gamma interferon from inclusion bodies. Journal of Biotechnology. 1996, 52, (2), 127-133.

Cing-Yuen Won.; Maurice R. Eftin. Incorporation of Tryptophan Analogues into Staphylococcal Nuclease, Its V66W Mutant, and Δ137-149 Fragment:? Spectroscopic Studies. Biochemistry. 1998, 37, (25), 8938-8946.

GHS Hazard and Precautionary Statements

Hazard Statements: H302-H315-H319-H332-H500

Harmful if swallowed. Causes skin irritation. Causes serious eye irritation. Harmful if inhaled. May form combustible dust concentrations in air

Precautionary Statements: P261-P264b-P270-P271-P280-P301+P312-P302+P352-P304+P340-P305+P351+P338-P312-P330-P332+P313-P337+P313-P362-P501c

Avoid breathing dust/fume/gas/mist/vapours/spray. Wash face, hands and any exposed skin thoroughly after handling Do not eat, drink or smoke when using this product. Use only outdoors or in a well-ventilated area. Wear protective gloves/protective clothing/eye protection/face protection. IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell. IF ON SKIN: Wash with plenty of soap and water. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Rinse mouth. If skin irritation occurs: Get medical advice/attention. If eye irritation persists: Take off contaminated clothing and wash before reuse. Dispose of contents/ container to an approved waste disposal plant

Other References

Merck
14,4562
Beilstein
3591990
Harmonized Tariff Code
2925.29
TSCA
Yes
RTECS
MF4300000

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