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60-24-2 - 2-Mercaptoethanol, Electrophoresis Grade, 98+% - Thioethylene glycol - Thioglycol - J66742 - Alfa Aesar

J66742 2-Mercaptoethanol, Electrophoresis Grade, 98+%

CAS Number
60-24-2
Synonyms
Thioethylene glycol
Thioglycol

Size Price ($) Quantity Availability
250g 23.80
1000g 64.90
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2-Mercaptoethanol, Electrophoresis Grade, 98+%

MDL
MFCD00004890
EINECS
200-464-6

Chemical Properties

Formula
C2H6OS
Formula Weight
78.13
Form
Liquid
Melting point
-100°
Boiling Point
156-158°
Flash Point
73°(163°F)
Density
1.114
Sensitivity
Air Sensitive & Hygroscopic
Solubility
Fully miscible in water. Soluble in alcohol, ether, benzene, and other organic solvents.

Applications

β-Mercaptoethanol can act as an enzyme reactivator in systems necessitating reduction for activation, and has been commonly used to reduce disulfide bonds in order to separate protein subunits for use in electrophoresis. This product can also be used to form o-Phthalaldehyde-beta-mercaptoethanol (OPAME), a fluorogenic reagent shown to detect amino acids based on its reaction with primary amines. More recently OPAME has been used to distinguish between ssDNA/dsDNA and even single base mismatches in the hybridization of DNA. It is widely used for retarding oxidation of biological compounds in solution.

Notes

Handle under dry protective gas. Store in cool condition. Incompatible with air, moisture, oxidizing agents.

Literature References

Margaret L. Heidrick..; Linda C. Hendricks.; David E. Cook. Effect of dietary 2-mercaptoethanol on the life span, immune system, tumor incidence and lipid peroxidation damage in spleen lymphocytes of aging BC3F1 mice. Mechanisms of Ageing and Development. 1984, 27 (3),341-358 .

B. Tasheva.; G. Dessev. Artifacts in sodium dodecyl sulfate-polyacrylamide gel electrophoresis due to 2-mercaptoethanol.Anal. Biochem. 1983, 129 (1),98-102 .

GHS Hazard and Precautionary Statements

Hazard Statements: H301-H310-H315-H317-H335-H318-H400-H410-H227

Toxic if swallowed. Fatal in contact with skin. Causes skin irritation. May cause an allergic skin reaction. May cause respiratory irritation. Causes serious eye damage. Very toxic to aquatic life. Very toxic to aquatic life with long lasting effects. Combustible liquid.

Precautionary Statements: P210-P261-P262-P273-P301+P310-P305+P351+P338-P361-P302+P350-P405-P501a

Keep away from heat/sparks/open flames/hot surfaces. - No smoking. Avoid breathing dust/fume/gas/mist/vapours/spray. Do not get in eyes, on skin, or on clothing. Avoid release to the environment. IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Remove/Take off immediately all contaminated clothing. IF ON SKIN: Gently wash with plenty of soap and water. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Other References

Merck
14,5869
Beilstein
773648
Hazard Class
6.1
Packing Group
II
Harmonized Tariff Code
2930.90
TSCA
Yes
RTECS
KL5600000

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