Cavity size is the major determinant as to which cyclodextrin is used in complexation. The cavity diameter of β-cyclodextrins or β-glucopyranose unit compounds is well-suited for use with molecules the size of hormones, vitamins and many compounds frequently used in tissue and cell culture applications. For this reason, β-cyclodextrin is most commonly used as a complexing agent. It is used to increase the solubility of non-polar substances such as fatty acids, lipids, vitamins and cholesterol for use in cell culture applications. Methyl-β-cyclodextrin is used to remove cholesterol from cultured cells and is employed to remove lipid rafts.
Yannis Dotsikas.; Yannis L Loukas. Kinetic degradation study of insulin complexed with methyl-beta cyclodextrin. Confirmation of complexation with electrospray mass spectrometry and 1H NMR. J. Pharm. Biomed. Anal. 2002, 29 (3),487-494 .