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BAC is used as a reversible cross-linker for polyacrylamide gels, to simplify isolation of macromolecules separated on the gels. The disulfide linkage can be broken with a suitable reducing agent. A 1-g slice of a 4% (12:1) gel will dissolve in 5 mL of 100 mM DTT in TBE (pH 8.3) in less than 30 min. The pH of 8.3 is optimum for rapid dissolution of the gel.
Haruyuki Hiratani.; Carmen Alvarez-Lorenzo.; Jeffrey Chuang.; Orhan Guney.; Alexander Yu. Grosberg.; Toyoichi Tanaka. Effect of Reversible Cross-linker, N,N-Bis(acryloyl)cystamine, on Calcium Ion Adsorption by Imprinted Gels.Langmuir. 2001, 17 (14), 4431-4436 .
Hua Yu.; David W. Grainger. Amphiphilic Thermosensitive N-Isopropylacrylamide Terpolymer Hydrogels Prepared by Micellar Polymerization in Aqueous Media.Macromolecules . 1994, 27 (16),4554-4560 .
Hazard Statements: H315-H319-H335
Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.
Precautionary Statements: P261-P264b-P271-P280-P302+P352-P304+P340-P305+P351+P338-P312-P332+P313-P337+P313-P362-P501c
Avoid breathing dust/fume/gas/mist/vapours/spray. Wash face, hands and any exposed skin thoroughly after handling Use only outdoors or in a well-ventilated area. Wear protective gloves/protective clothing/eye protection/face protection. IF ON SKIN: Wash with plenty of soap and water. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Call a POISON CENTER or doctor/physician if you feel unwell. If skin irritation occurs: Get medical advice/attention. If eye irritation persists: Take off contaminated clothing and wash before reuse. Dispose of contents/ container to an approved waste disposal plant