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Colloidal gold is an electron-dense, non-fading marker useful as a probe in electron microscopy, light microscopy and immunoblotting. It requires no additional processing for detection, but in some applications the signal can be enhanced by reaction with silver. It can be complexed with biomolecules by strong, non-covalent interactions. Particles less than 15 nm are most useful in transmission electron microscopy and for applications where access to the probe may be hindered by larger particles.
J.F. Pierson; D. Wiederkehr; A. Billard. Reactive magnetron sputtering of copper, silver, and gold.Thin Solid Films.,2005,478 (1-2), 196-205.
Mathias Brust, et al. Synthesis of thiol-derivatised gold nanoparticles in a two-phase Liquid-Liquid system.J. Chem. Soc., Chem. Commun.,1994,(7), 801-802.