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It has been used in tissue culture media. It is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. It can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 100mg/mL. Kanamycin can be used as a selection agent in several types of isolation media.
Gabriela Antu´nez de Mayolo, et. al. Moxalactam as a counter-selection antibiotic for Agrobacterium mediated transformation and its positive effects on Theobroma cacaosomatic embryogenesis.Plant Sci.,2003,164607-615.
Misumi, M and Tanaka, N. Mechanism of inhibition of translocation by kanamycin and viomycin: A comparative study with fusidic acid.Biochem Biophys Res Commun.,1980,92(2), 647-654.
Hazard Statements: H317-H334-H360FD
May cause an allergic skin reaction. May cause allergy or asthma symptoms or breathing difficulties if inhaled. May damage fertility. Suspected of damaging the unborn child. May damage fertility. May damage the unborn child.
Precautionary Statements: P201-P202-P261-P272-P280g-P281-P285-P302+P352-P304+P341-P308+P313-P333+P313-P342+P311-P363-P501c
Obtain special instructions before use. Do not handle until all safety precautions have been read and understood. Avoid breathing dust/fume/gas/mist/vapours/spray. Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves. Use personal protective equipment as required. In case of inadequate ventilation wear respiratory protection. IF ON SKIN: Wash with plenty of soap and water. IF INHALED: If breathing is difficult, remove to fresh air and keep at rest in a position comfortable for breathing. IF exposed or concerned: Get medical advice/attention. If skin irritation or rash occurs: If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician. Wash contaminated clothing before reuse. Dispose of contents/ container to an approved waste disposal plant