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It has been used in tissue culture media. It is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. It can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 100mg/mL. Kanamycin can be used as a selection agent in several types of isolation media.
Gabriela Antu´nez de Mayolo, et. al. Moxalactam as a counter-selection antibiotic for Agrobacterium mediated transformation and its positive effects on Theobroma cacaosomatic embryogenesis.Plant Sci.,2003,164607-615.
Misumi, M and Tanaka, N. Mechanism of inhibition of translocation by kanamycin and viomycin: A comparative study with fusidic acid.Biochem Biophys Res Commun.,1980,92(2), 647-654.
Hazard Statements: H334-H317-H360
May cause allergy or asthma symptoms or breathing difficulties if inhaled. May cause an allergic skin reaction. May damage fertility or the unborn child.
Precautionary Statements: P284-P201-P261-P280-P405-P501a
Wear respiratory protection. Obtain special instructions before use. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.