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31282-04-9 - Hygromycin B, 50 mg/ml in distilled water, sterile-filtered - J67371 - Alfa Aesar

J67371 Hygromycin B, 50 mg/ml in distilled water, sterile-filtered

CAS Number
31282-04-9
Synonyms

Size Price ($) Quantity Availability
1ml 30.90
5x1ml 124.00
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Hygromycin B, 50 mg/ml in distilled water, sterile-filtered

MDL
MFCD06795479
EINECS
250-545-5

Chemical Properties

Form
Liquid
Solubility
Fully miscible in water and also soluble in methanol or ethanol and buffer solution.

Applications

Hygromycin B was used for hygro resistance effect in the following cells transfected 293T cells with mixture of plasmids at 160 ?g/ml, MCF-7ALOX12 cells at 150 ?g/ml, MSCV Luciferase PGK-hygro plasmid and STAT3 Reporter HeLa Stable Cell Line (stably transfected with Luciferase gene under the control of STAT3 promoter) maintained in DMEM supplemented with 10% FBS. It is suitable for the selection of cells transfected with Escherichia coli hygromycin resistance gene (hyg or hph), as well as for the maintenance of the Hygromycin phenotype of resistant cells. Consistent with its more specific effect on translocation, hygromycin B exhibits monophasic inhibition of polypeptide synthesis, suggesting that it binds to a single site on the ribosome.

Notes

Protect from heat. Keep the container tightly closed and place it in a cool, dry and well ventilated condition. Store in freezer at -20°C.

Literature References

Rao RN, et. al. Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli.Antimicrob Agents Chemother.,1983,24(5), 689-95.

González A, E, et al. Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes.Biochim Biophys Acta.,1978,521(2), 459-69.

GHS Hazard and Precautionary Statements

Hazard Statements: H301-H310-H330-H318-H334-H317

Toxic if swallowed. Fatal in contact with skin. Fatal if inhaled. Causes serious eye damage. May cause allergy or asthma symptoms or breathing difficulties if inhaled. May cause an allergic skin reaction.

Precautionary Statements: P301+P310a-P304+P340-P305+P351+P338-P320-P330-P405-P501a

IF SWALLOWED: IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Specific treatment is urgent (see label). Rinse mouth. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.

Other References

Hazard Class
6.1
Packing Group
II
Harmonized Tariff Code
2941.90
TSCA
No
RTECS
WK2130000

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