Cookies disclaimer

I agree Our site saves small pieces of text information (cookies) on your device in order to deliver better content and for statistical purposes. You can disable the usage of cookies by changing the settings of your browser. By browsing our website without changing the browser settings you grant us permission to store that information on your device.

J67371 Hygromycin B, 50 mg/ml in distilled water, sterile-filtered

CAS Number
31282-04-9
Synonyms

Stock No. Size Price ($) Quantity Availability
J67371-XF 1ml 69.90
J67371-8= 5x1ml 204.00
Add to Cart Bulk/Specialty Print Quote View Item

Hygromycin B, 50 mg/ml in distilled water, sterile-filtered

MDL
MFCD06795479
EINECS
250-545-5

Chemical Properties

Form
Liquid
Storage & Sensitivity
Store at -20°C.
Solubility
Fully miscible in water and also soluble in methanol or ethanol and buffer solution.

Applications

Hygromycin B was used for hygro resistance effect in the following cells transfected 293T cells with mixture of plasmids at 160 ?g/ml, MCF-7ALOX12 cells at 150 ?g/ml, MSCV Luciferase PGK-hygro plasmid and STAT3 Reporter HeLa Stable Cell Line (stably transfected with Luciferase gene under the control of STAT3 promoter) maintained in DMEM supplemented with 10% FBS. It is suitable for the selection of cells transfected with Escherichia coli hygromycin resistance gene (hyg or hph), as well as for the maintenance of the Hygromycin phenotype of resistant cells. Consistent with its more specific effect on translocation, hygromycin B exhibits monophasic inhibition of polypeptide synthesis, suggesting that it binds to a single site on the ribosome.

Notes

Protect from heat. Keep the container tightly closed and place it in a cool, dry and well ventilated condition. Store in freezer at -20°C.

Literature References

Rao RN, et. al. Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli.Antimicrob Agents Chemother.,1983,24(5), 689-95.

González A, E, et al. Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes.Biochim Biophys Acta.,1978,521(2), 459-69.

GHS Hazard and Precautionary Statements

Hazard Statements: H301-H310-H317-H318-H331-H334

Toxic if swallowed. Fatal in contact with skin. May cause an allergic skin reaction. Causes serious eye damage. Toxic if inhaled. May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary Statements: P261-P262-P264b-P270-P271-P272-P280-P285-P301+P310-P302+P350-P304+P340-P305+P351+P338-P310-P311-P330-P333+P313-P361-P363-P501c

Avoid breathing dust/fume/gas/mist/vapours/spray. Do not get in eyes, on skin, or on clothing. Wash face, hands and any exposed skin thoroughly after handling Do not eat, drink or smoke when using this product. Use only outdoors or in a well-ventilated area. Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves/protective clothing/eye protection/face protection. In case of inadequate ventilation wear respiratory protection. IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician. IF ON SKIN: Gently wash with plenty of soap and water. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Call a POISON CENTER or doctor/physician. Rinse mouth. If skin irritation or rash occurs: Get medical advice/attention. Remove/Take off immediately all contaminated clothing. Wash contaminated clothing before reuse. Dispose of contents/ container to an approved waste disposal plant

Other References

Hazard Class
6.1
Packing Group
III
Harmonized Tariff Code
2941.90
TSCA
No
RTECS
WK2130000

Recommended

  • J61278

    Puromycin dihydrochloride, 99%
  • J67140

    Zeocin™, 100 mg/ml in HEPES, sterile-filtered

Recently Viewed

Chemicals

Life Science

Metals & Materials

Catalysts

Analytical & Labware