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Hygromycin B was used for hygro resistance effect in the following cells transfected 293T cells with mixture of plasmids at 160 ?g/ml, MCF-7ALOX12 cells at 150 ?g/ml, MSCV Luciferase PGK-hygro plasmid and STAT3 Reporter HeLa Stable Cell Line (stably transfected with Luciferase gene under the control of STAT3 promoter) maintained in DMEM supplemented with 10% FBS. It is suitable for the selection of cells transfected with Escherichia coli hygromycin resistance gene (hyg or hph), as well as for the maintenance of the Hygromycin phenotype of resistant cells. Consistent with its more specific effect on translocation, hygromycin B exhibits monophasic inhibition of polypeptide synthesis, suggesting that it binds to a single site on the ribosome.
Rao RN, et. al. Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli.Antimicrob Agents Chemother.,1983,24(5), 689-95.
González A, E, et al. Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes.Biochim Biophys Acta.,1978,521(2), 459-69.
Hazard Statements: H301-H310-H330-H318-H334-H317
Toxic if swallowed. Fatal in contact with skin. Fatal if inhaled. Causes serious eye damage. May cause allergy or asthma symptoms or breathing difficulties if inhaled. May cause an allergic skin reaction.
Precautionary Statements: P301+P310a-P304+P340-P305+P351+P338-P320-P330-P405-P501a
IF SWALLOWED: IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Specific treatment is urgent (see label). Rinse mouth. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.