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Hygromycin B was used for hygro resistance effect in the following cells transfected 293T cells with mixture of plasmids at 160 ?g/ml, MCF-7ALOX12 cells at 150 ?g/ml, MSCV Luciferase PGK-hygro plasmid and STAT3 Reporter HeLa Stable Cell Line (stably transfected with Luciferase gene under the control of STAT3 promoter) maintained in DMEM supplemented with 10% FBS. It is suitable for the selection of cells transfected with Escherichia coli hygromycin resistance gene (hyg or hph), as well as for the maintenance of the Hygromycin phenotype of resistant cells. Consistent with its more specific effect on translocation, hygromycin B exhibits monophasic inhibition of polypeptide synthesis, suggesting that it binds to a single site on the ribosome.
Rao RN, et. al. Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli.Antimicrob Agents Chemother.,1983,24(5), 689-95.
González A, E, et al. Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes.Biochim Biophys Acta.,1978,521(2), 459-69.
Hazard Statements: H301-H310-H317-H318-H331-H334
Toxic if swallowed. Fatal in contact with skin. May cause an allergic skin reaction. Causes serious eye damage. Toxic if inhaled. May cause allergy or asthma symptoms or breathing difficulties if inhaled.
Precautionary Statements: P261-P262-P264b-P270-P271-P272-P280-P285-P301+P310-P302+P350-P304+P340-P305+P351+P338-P310-P311-P330-P333+P313-P361-P363-P501c
Avoid breathing dust/fume/gas/mist/vapours/spray. Do not get in eyes, on skin, or on clothing. Wash face, hands and any exposed skin thoroughly after handling Do not eat, drink or smoke when using this product. Use only outdoors or in a well-ventilated area. Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves/protective clothing/eye protection/face protection. In case of inadequate ventilation wear respiratory protection. IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician. IF ON SKIN: Gently wash with plenty of soap and water. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Call a POISON CENTER or doctor/physician. Rinse mouth. If skin irritation or rash occurs: Get medical advice/attention. Remove/Take off immediately all contaminated clothing. Wash contaminated clothing before reuse. Dispose of contents/ container to an approved waste disposal plant