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Contains: sodium chloride (137.93mM), sodium hydrogen phosphate heptahydrate (8.06mM), potassium chloride (2.67mM), potassium dihydrogen phosphate (1.47mM), and magnesium chloride hexahydrate (0.493mM), pH 7.4.
To make 1X, dilute 10-fold with DI water and the pH of the 1X should be 7.4 ± 0.2.
In addition, since CaCl2 and MgCl2 tend to precipitate at a high concentration in 10X solution, a mixture of CaCl2 and MgCl2 solutions (1000X) is provided in a separate bottle. 1mL of this mixture should be added to 1L of 1X diluted PBS.