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J75831 Phenol:Chloroform:Isoamyl Alcohol (25:24:1), Molecular Biology Grade, Ultrapure, Thermo Scientific

CAS Number

Stock No. Size Price ($) Quantity Availability
J75831-AE 100ml 79.60
J75831-AN 400ml 147.00
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Phenol:Chloroform:Isoamyl Alcohol (25:24:1), Molecular Biology Grade, Ultrapure, Thermo Scientific

Chemical Properties

Storage & Sensitivity
Keep Cold.


Nucleic acid extraction and purification.


A liquid mixture of phenol, ACS Reagent Grade chloroform and ACS Reagent Grade isoamyl alcohol in a ratio of 25:24:1 supplied with an additional Alkaline Equilibration Buffer used to adjust the pH to 8.0.

Phenol:Chloroform:Isoamyl Alcohol (25:24:1) is packaged at pH 6.7, the product is accompanied by a separate Alkaline Equilibration Buffer.

When ready to use add the supplemental buffer's entire contents to the Phenol:Chloroform:Isoamyl Alcohol, then mix or shake, and allow to separate. Once this is completed the product will be at a pH of 8.0.

Other References

Harmonized Tariff Code


  • B20786

    7-Nitroindole-2-carboxylic acid, 96%
  • H26003

    1H-Benzotriazole-4-sulfonic acid, 97%
  • H31505

    1-Phenylsulfonyl-7-azaindole-3-boronic acid pinacol ester, 95%
  • 14835

    Formamide, ACS, 99.5+%
  • L08391

    Naphthalene-2-carboxamide, 98%

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