I agree Our site saves small pieces of text information (cookies) on your device in order to deliver better content and for statistical purposes. You can disable the usage of cookies by changing the settings of your browser. By browsing our website without changing the browser settings you grant us permission to store that information on your device.
Nucleic acid extraction and purification.
A liquid mixture of phenol, ACS Reagent Grade chloroform and ACS Reagent Grade isoamyl alcohol in a ratio of 25:24:1 supplied with an additional Alkaline Equilibration Buffer used to adjust the pH to 8.0.
Phenol:Chloroform:Isoamyl Alcohol (25:24:1) is packaged at pH 6.7, the product is accompanied by a separate Alkaline Equilibration Buffer.
When ready to use add the supplemental buffer's entire contents to the Phenol:Chloroform:Isoamyl Alcohol, then mix or shake, and allow to separate. Once this is completed the product will be at a pH of 8.0.